Preparing Saline Solution

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Preparing Saline Solution - Michigan Medicine

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Preparing Saline Solution Instructions - 2 - Disclaimer: This document contains information and/or instructional materials developed by the University of Michigan Health System (UMHS) for the typical patient with your condition. It may include links to online content that was not created by UMHS and for which UMHS does not assume responsibility.

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Protocol 3: Immunofluorescence on Frozen Sections

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NaOH Solution (1N, 40g/liter) Gelatin solution: 1g gelatin + 1L ddH 2 O + 0.1g chromium potassium sulfate . Procedure . 1. Soak slides in NaOH for at least 15 minutes 2. Rinse slides in ddH 2 O one time fast, then once fo r 15 minutes 3. Dip slides in gelatin solutoi n 4. Air-dry slides 5. Store slides in slide boxes at room temperature

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Propidium Iodide Nucleic Acid Stain

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4.1 Make a 3 μM solution of PI by diluting the 1 mg/mL (1.5 mM) stock solution 1:500 in staining buffer (100 mM Tris, pH 7.4, 150 mM NaCl, 1 mM CaCl 2, 0.5 mM MgCl 2, 0.1% Nonidet P-40). A 1 mL volume will be required for each cell sample. 4.2 Centrifuge the cell suspension from step 3.3, discard the supernatant, tap to loosen the pellet,

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Preparing Saline Solution