Search results with tag "Tca precipitation"
Ni-NTA Purification System - Thermo Fisher Scientific
tools.thermofisher.comprotocol on page 17 or hybrid protocol on page 19. Note: To perform SDS-PAGE with samples in Guanidinium Lysis Buffer, you need to dilute the samples, dialyze the samples, or perform TCA precipitation prior to SDS-PAGE to prevent the precipitation of SDS. Harvesting Insect Cells
Fundamentals of Protein Chemistry - Duke University
genome.duke.eduTCA Precipitation: (if [protein] is >0.05 mg/mL) Chill protein in a microcentrifuge tube to 0°C . Add 1/9 volume of cold 100% w/v TCA. Vortex . Incubate at 0°C for 30-60 min. Spin down in microcentrifuge. Remove supernatant. Wash pellet 3x w/ 200 μL cold acetone (do not vortex)
Ni-NTA Purification System - Thermo Fisher Scientific
tools.thermofisher.comprotocol on page 17 or hybrid protocol on page 19. Note: To perform SDS-PAGE with samples in Guanidinium Lysis Buffer, you need to dilute the samples, dialyze the samples, or perform TCA precipitation prior to SDS-PAGE to prevent the precipitation of SDS. Harvesting Insect Cells