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Quant-iT™ PicoGreen ® dsDNA Reagent and Kits
tools.thermofisher.comDynamic range and sensitivity of the Quant-iT™ PicoGreen® dsDNA assay. Calf thymus DNA was added to cuvettes containing Quant-iT™ PicoGr een® dsDNA reagent diluted in 10 mM Tris-HCl, 1 mM EDTA, pH 7.5 (TE). The samples were excited at 480 nm and the fluo res cence emission intensity was measured at 520 nm using a spectrofluorometer.