Search results with tag "Hplc phases"
GOOD LABORATORY PRACTICE for HPLC
www.ispybio.comWhen separating mixtures containing acids and/or bases by reversed phase HPLC, it is necessary to control the pH of the mobile phase using an appropriate buffer in order to achieve reproducible results. Example Chromatographic separation where resolution goes from an unacceptable 1.4
Reversed-phase HPLC Bu ers
ccc.chem.pitt.edu9 Reversed-phase HPLC Bu!ers High-quality bu!ers (solutions, solids or concentrates) Shyam Verma shyam.verma@sial.com Consideration of the a!ects of pH on analyte retention,
HPLC Troubleshooting Guide
hplc.euproblems observed in reversed-phase HPLC (RP-HPLC) separations. Four major problem areas are covered: peak shape, retention time changes, ghost peaks and problems related to column backpressure. In addition, a section on column care is included – procedures that will help you get maximum lifetimes from your columns.