Phase hplc
Found 8 free book(s)
GOOD LABORATORY PRACTICE for HPLC
www.ispybio.comWhen separating mixtures containing acids and/or bases by reversed phase HPLC, it is necessary to control the pH of the mobile phase using an appropriate buffer in order to achieve reproducible results. Example Chromatographic separation where resolution goes from an unacceptable 1.4
HPLC Troubleshooting Guide
hplc.euproblems observed in reversed-phase HPLC (RP-HPLC) separations. Four major problem areas are covered: peak shape, retention time changes, ghost peaks and problems related to column backpressure. In addition, a section on column care is included – procedures that will help you get maximum lifetimes from your columns.
Tips for practical HPLC analysis
www.shimadzu.eu.comHPLC-type acetonitrile has the lowest absorbance (particularly for short wavelengths). Using an organic solvent with lower absorbance as the mobile phase results in less noise in UV detection, and so HPLC-type acetonitrile is suitable for high-sensitivity analysis in the UV short-wavelength range. Also, using HPLC-type acetonitrile
Waters UPLC, UHPLC, and HPLC Column Selection and Mobile ...
www.waters.comUsing a wide mobile-phase pH range is an effective approach to change compound selectivity. Increase selectivity for: - Acids (Peaks 3 and 6) - Bases (Peaks 1 and 4) Neutrals (Peaks 2 and 5) are largely unaffected by mobile-phase pH. The pH of the mobile phase has the greatest impact on analyte retention.
High Performance Liquid Chromatography (HPLC)
www2.chemistry.msu.eduGradient Elution in RP-HPLC Nonpolar stationary phase. To elute solutes, must increase the eluent strength by adding organic solvent (mixed with water). Solvent plays a key role in separation!!!! Make the solutes more soluble in mobile phase than in stationary phase!!! Solution to the general elution problem.
A Guide to Validation in HPLC - PARAS'S PHARMACY WORLD
parasshah.weebly.comphase. For absorbance detectors, longer path lengths in the flow cell enhances sensitivity though often to the detriment of post column dispersion. ... For an HPLC system this would involve the reproducibility of a number of replicate injections (ie 6) of an analytical solution.
HPLC Troubleshooting Cover
ccc.chem.pitt.eduHPLC Troubleshooting Uwe D. Neue, Waters Corporation 1. Column Life-Time 2. Variable Retention Times 3. Drifting Retention Times 4. Column-to-Column and Batch-to-Batch Reproducibility 5. Sample Preparation Problems 6. Sources of Peak Tailing 7. Normal-Phase Chromatography 8. System Volume, Dead Volume, Dwell Volume 9. Transfer of Gradient ...
Tips and Tricks for HPLC and UHPLC
assets.thermofisher.com•Solvent compatibility • Try to use pre-mixed solvents • Add 5-10% of organic eluent to the aqueous eluent • Add 5-10% aqueous eluent to the organic eluent • Avoids local crystallization in the pump (with buffers) • Eluents with salt buffers • Change eluents with salt buffers regularly • Filtrate buffers • Use water with 18,2M Ohm AND <5ppb TOC