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Real-time PCR handbook - Faculdade de Filosofia, Ciências ...
www.ffclrp.usp.brThe change in fl uorescence over the course of the reaction is measured by an instrument that combines thermal cycling with fl uorescent dye scanning capability. By plotting fl uorescence against the cycle number, the real-time PCR instrument generates an amplifi cation plot that represents the accumulation of product over the
Lipophilic Tracers—Dil, DiO, DiD, DiA, and DiR
tools.thermofisher.comApproximate fl uorescence excitation/emission maxima: See Table 2. Lipophilic Tracers—Dil, DiO, DiD, DiA, and DiR | 2 staining is usually less intense than that of DiI, and occasionally fails completely in fixed tissues.6,11 DiD (D307, D7757) is an analog of DiI with markedly red-shifted fluorescence excitation