Search results with tag "Stock solutions"
Protocols and tips in protein purification
www.sheffield.ac.ukIt is best to keep stock buffers in the fridge. Preparation of working buffers from stock solutions By having stock solutions you can prepare any buffer for protein purification in seconds. Simply pour required volumes of stock solutions in to a Duran bottle and adjust the volume with ultra pure water to the top mark on the bottle.
Roche Applied Science The Complete Guide for Protease ...
iti.stanford.eduSoluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2-8°C or at least 12 weeks at -15 to -25°C. Can be used in thiol-contain-ing solutions at room temperature. Soluble in aqueous buffers, or add
Recipes for Reagents and Stock Solutions - GREENOMES
greenomes.orgRecipes for Reagents and Stock Solutions The success of the laboratories depends on the use of high-quality reagents. Follow the recipes with care and pay attention to cleanliness. Use a clean spatula for each ingredient or carefully pour each ingredient from its bottle. I. DNA Isolation 4.4 M Ammonium Acetate (NH 4OAc) Edward's Buffer
HisTrap HP, 1 ml and 5 ml - Auburn University
webhome.auburn.edustock solutions; by diluting the sample with binding buffer; or by buffer exchange, (see Table 4). Do not use strong bases or acids for pH-adjustment (precipitation risk). Filter the sample through a 0.22 µm or a 0.45 µm filter and/or centrifuge it immediately before applying it to the column.