Actinomycete Isolation Agar

1 Please refer disclaimer Isolation AgarM490 Actinomycete Isolation agar is used for Isolation and propagation of Actinomycetes from soil and **IngredientsGms / LitreSodium pH ( at 25 C) **Formula adjusted, standardized to suit performance parametersDirectionsSuspend grams in 1000 ml distilled water containing 5 ml glycerol. Heat to boiling to dissolve the medium as desired. Sterilize by autoclaving at 15 lbs pressure (121 C) for 15 And InterpretationActinomycetes are gram-positive bacteria, which show marked chemical and morphological diversity but form a distinctevolutionary line of organisms that range from coccoid and pleomorphic forms to branched filaments (1). Actinomycetesform an integral part of soil, water and vegetation. Actinomycete development leads to the formation of volatile metabolites(2). Traces of these volatile metabolites are sufficient to impart disagreeable odour to water or a muddy flavour to fish(3).

2 Actinomycetes also cause disruptions in wastewater treatment by forming massive growths, which are capable ofproducing thick foam in the activated sludge process (4, 5). Actinomyces Isolation agar used for Isolation and propagation ofActinomycetes from soil and water was formulated by Olsen (6). Actinomycete Isolation agar contains sodium caseinate as nitrogen source. Asparagine in addition to being an amino acid is alsoa source of nitrogen. Sodium propionate is used as a substrate in anaerobic fermentation. Dipotassium phosphate provides thebuffering system. The sulphates serve as source of sulphur and metallic ions. Glycerol serves as an additional source of the plates with 1 drop of diluted culture or specimen and spread over the surface using a sterile bent glass rod. Incubateat 35-37 C for 40-72 hours. The media can be used for long term storage after sufficient growth is obtained. agar slants areused for maintenance of cultures over a shorter period of ControlAppearanceCream to yellow homogeneous free flowing powderGellingFirm, comparable with agar gelColour and Clarity of prepared mediumYellow to light amber coloured opalescent gel forms in Petri platesReactionReaction of w/v aqueous solution containing glycerol at 25 C.

3 PH : ResponseM490: Cultural characteristics observed after an incubation at 35-37 C for 40-72 LaboratoriesTechnical DataNocardia asteroides ATCC19427good-luxuriantEscherichia coli ATCC25922inhibitedStreptomyces albus subspalbus ATCC 3004good-luxuriantStreptomyces lavendulaeATCC 19247good-luxuriantStorage and Shelf LifeStore below 30 C in tightly closed container and the prepared medium at 2 - 8 C. Use before expiry date on Collee J. G., Fraser A. G., Marmion B. P., Simmons A., (Eds.), Mackie and McCartney, Practical Medical Microbiology,1996, 14th Edition, Churchill Adams B. A., 1929, Water and Water Eng., 31 Eaton A. D., Clesceri L. S. and Greenberg A. W., (Eds.), 2005, Standard Methods for the Examination of Water andWastewater, 21st Ed., APHA, Washington, Lechevalier H. A., 1975, Environ. Protection Technol. Ser., EPA-600/ 2-75-031, U. S. Environmental Protection Agency,Cincinnati, Lechevalier M.

4 P., and Lechevalier H. A., 1974, Int. J. , 24 Olsen, 1960, Personal : 2 / 2015 HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-61471919 Email: :User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.