AGILENT GPC/SEC POLYMER STANDARDS

1 AGILENT GPC/SEC POLYMER STANDARDS2 ContentsPOLYMER STANDARDS FOR GPC/SEC 3 InfinityLab EasiVial 5 InfinityLab EasiCal 8 Polystyrene STANDARDS 9 Polymethylmethacrylate STANDARDS 11 Polyethylene glycol/oxide STANDARDS 13 Polyacrylic acid STANDARDS 15 TECHNIQUE REVIEW 16 AGILENT Publications 18 AGILENT GCP/SEC Analysis systems 19 EasiCal standardsNew format shortens sample preparation time and the speed of calibration1993 PLgel columns, individual STANDARDS , and standard kitsPolymer Laboratories founded to develop market-leading products for organic GPC/SECPLgel MIXED columns, PL aquagel columnsMIXED columns improve data quality.

2 And novel chemistries for analysis of water-soluble polymersGPC softwareDedicated software streamlines GPC/SEC calculations198119841976 PL-GPC 220 instrumentMarket-leading high temperature GPC system for even the most difficult samples at temperatures up to 220 C19993 AGILENT manufactures the widest range of POLYMER STANDARDS on the market. These STANDARDS are critical for generating accurate results on: GPC/SEC systems Viscometers Light scattering systemsPolymer STANDARDS are available in powder form , and in prepared InfinityLab EasiVial and EasiCal formats, which save time by eliminating tedious weighing procedures in the molecular weight (MW) STANDARDS are available in 1, 5, and 10 g quantities from AGILENT , for further use as model polymers in research and analytical AGILENT STANDARDS are manufactured, under ISO 9001:2008 approved quality system. Each is fully traceable with a unique batch number and a complete certification of analysis (COA).

3 Finally, all COAs include details of the exact method and characterization results for maximum transparency and STANDARDS FOR GPC/SECThe 1260 Infinity II Multi-Detector GPC/SEC System The first choice for accurate, reproducible POLYMER analysis. Select any combination of light scattering, viscometry and refractive index detection for absolute molecular weights and columns and EasiVial standardsNew chemistries deliver high-pore-volume materials for increased resolution, and EasiVial STANDARDS simplify calibration procedures even further2004 PLgel Olexis columnsOptimized for polyolefin analysis with highest resolution and data quality for even ultrahigh molecular weight samples20071260 Infinity Multi Detector Suite and PolarGel columnsThe 1260 Infinity MDS turns any LC into a powerful multi-detector GPC system, and PolarGel columns analyze polar samples in any solvent system2009PL MultiSolvent GPC columnsThe newest addition to the InfinityLab GPC family, offering solvent flexibility for a variety of GPC analyses all on one provides the widest range of MW STANDARDS on the market, from 162 to 15 million g/mol.

4 Even at the highest MWs, AGILENT STANDARDS are made with the utmost precision, and the polydispersity remains This low polydispersity means that distorted peaks are easily identified before they can skew the calibration and the measurement. Individual STANDARDS are generally available in 1, 5, and 10 g GPC/SEC column calibration, AGILENT provides kits covering both a wide range of MWs and a wide variety of solvents. Each kit has been preselected to generate an even distribution of points across the chosen MW POLYMER in the kit is individually certified, and all necessary data is provided to generate a calibration curve straight out of the box. For more information on choosing the correct STANDARDS for a particular eluent, see page leading AGILENT GPC and SEC columns have set the standard for reliability, speed, and performance for over 40 years: Organic GPC/SEC columns, publication 5990-7994EN Aqueous and polar GPC/SEC columns, publication 5990-7995 ENUnbeatable precision and varietyCalibration kits: for column and instrument calibrationStandards selection guidePolymer TypeIndividual MWCalibration KitsInfinityLab EasiCalInfinityLab EasiVialSolvent SystemUV/Vis SignalPolystyreneYe sYe sYe sYe sPrimary: OrganicStrongPolymethylmethacrylateYe sYe sYe sPrimary: Polar OrganicSecondary: OrganicStrongPolyethylene glycol/oxide (PEG/PEO)Ye sYe sYe sPrimary: AqueousSecondary: Polar OrganicWeakPolyacrylic acidYe sYe sPrimary: AqueousSecondary.

5 Polar OrganicStrongPOLYMER STANDARDS FOR GPC/SEC5 INFINITYLAB EASIVIALFor organic and aqueous GPC/SEC column calibration, InfinityLab EasiVial is the quickest and most convenient method to deliver an accurate 12-point column EasiVial kits are prepared sets of three vials, each containing four STANDARDS spaced across the full MW range of the kit. Simply add solvent and the STANDARDS are ready to use. With just one injection from each vial, a column is fully calibrated and ready for data kit contains 10 vials of each type (30 total) that are color coded for easy identification. Both 2 and 4 mL vials are available to suite most autosamplers. Appropriate InfinityLab EasiVial kits are available for all solvent systems: Polystyrene (PS), Polymethylmethacrylate (PMMA), and Polyethylene glycol/oxide (PEG/PEO).Ultra-narrow range MW STANDARDS allow easy identification of column degradation before it can alter add solvent Eliminates analyst hours wasted on tedious weighing procedures Widely applicable to room temperature, high temperature, and multidetector GPC Each vial contains equidistant peaks that are easily baseline resolvedConditionsColumns: 3 x PLgel 10 m MIXED-B, 7 5 x 300 mmEluent: THFFlow Rate: 1 0 mL/minTemp: 40 CDetector: PL-GPC 220 (RI)Peak Identification 1 6,035,000 2 483,000 3 19,720 4 1,260 Retention EasiVial PS-HTightly characterized STANDARDS eliminate error in the calibration curve for high accuracy measurements.

6 5 3,053,000 6 184,900 7 8,450 8 580 9 915,000 10 60,450 11 3,370 12 1626 INFINITYLAB EASIVIALS pecificationsInfinityLab EasiVial colorInfinityLab EasiVial PS-HInfinityLab EasiVial PS-MInfinityLab EasiVial PS-LInfinityLab EasiVial PMInfinityLab EasiVial PEG/PEOI nfinityLab EasiVial PEGN ominal Mp (g/mol)Red1,3001,0005802,00060028220,000 7,0003,00030,00013,0001,000500,00050,000 10,000300,000130,0007,0007,000,000500,00 050,0001,500,0001,500,00030,000 Yellow5803703701,00019419410,0003,0002,0 0013,0004,000600200,00030,0007,000130,00 070,0004,0003,000,000200,00030,0001,000, 0001,000,00020,000 Green1621621625001061065,0001,3001,0007, 0001,50040070,00013,0005,00070,00030,000 1,5001,000,000100,00020,000500,000500,00 013,000PS = polystyrene PM = polymethylmethacrylate H = STANDARDS to high molecular weight M = STANDARDS to medium molecular weight L = STANDARDS to low molecular weightAgilent InfinityLab Maximize Your LC Workflow EfficiencyHow can you make your LC workflow more efficient, so you can spend more time on your analytical priorities?

7 Find out with AGILENT InfinityLab an optimized portfolio of LC instruments, columns, and supplies designed to work together in perfect more at: www AGILENT com/chem/infinitylab7 INFINITYLAB EASIVIALO rdering informationInfinityLab EasiVial pre-weighed calibration kitsDescriptionVial Volume (mL)Quantity (Vials/Kit)Part No EasiVial PEG/PEO230PL2080-0201 EasiVial PEG/PEO430PL2080-0200 EasiVial PEG230PL2070-0201 EasiVial PEG430PL2070-0200 EasiVial PM230PL2020-0201 EasiVial PM430PL2020-0200 EasiVial PS-H230PL2010-0201 EasiVial PS-H430PL2010-0200 EasiVial PS-M230PL2010-0301 EasiVial PS-M430PL2010-0300 EasiVial PS-L230PL2010-0401 EasiVial PS-L430PL2010-0400 PEG/PEO Tri-Pack290PL2080-0202 PEG/PEO Tri-Pack490PL2080-0203 PEG Tri-Pack290PL2070-0202 PEG Tri-Pack490PL2070-0203 PMMA Tri-Pack290PL2020-0202 PMMA Tri-Pack490PL2020-0203PS-H Tri-Pack290PL2010-0202PS-H Tri-Pack490PL2010-0203PS-M Tri-Pack290PL2010-0302PS-M Tri-Pack490PL2010-0303PS-L Tri-Pack290PL2010-0402PS-L Tri-Pack490PL2010-0403 See alsoOrganic GPC/SEC columns, publication 5990-7994 ENAqueous and polar GPC/SEC columns.

8 Publication 5990-7995EN8 INFINITYLAB EASICALStir-in calibration STANDARDS Easy three-step process with no fuss Cost effective format saves money Only two injections for improved productivityThe InfinityLab EasiCal system for organic solvents consists of two different combs, each with ten detachable spatulas supporting a mixture of five POLYMER STANDARDS . The thin film of POLYMER (approximately 5 mg) on the tip of the PTFE spatulas rapidly dissolves when immersed in solvent to provide two GPC/SEC calibration solutions. A single pack provides ten spatulas of each type, with MW STANDARDS selected to provide equidistant calibration points for greater alsoOrganic GPC/SEC columns, publication 5990-7994 ENAqueous and polar GPC/SEC columns, publication 5990-7995 ENOrdering informationInfinityLab EasiCal pre-prepared polystyrene kitsPolystyrene PS-1 Part No PL2010-0501 (1/pk) Part No PL2010-0505 (5/pk)Polystyrene PS-2 Part No PL2010-0601 (1/pk) Part No PL2010-0605 (5/pk)Spatula A, Constituent Polymers Nominal Mp (g/mol)3,0001,30030,0005,000130,00020,00 0700,000100,0007,000,000400,000 Spatula B, Constituent Polymers Nominal Mp (g/mol)

9 58058010,0003,00070,00010,000300,00050,0 002,500,000200,000 Place one spatula of each type into appropriate volume of solventColumn calibration for GPC/SEC is as easy as 1, 2, 3 Step 1 Chromatograph each solution, only two injections requiredStep 2 Generate a 10 point calibration curveStep 3 9 POLYSTYRENE STANDARDSThe first choice standard for most organic applications Compatible with most organic solvents Certificate of Analysis meets international protocols Widest range of available MW STANDARDS for organic solventsPolystyrene STANDARDS are the first choice for many organic solvents for conventional GPC column calibration, and light scattering detectors and AGILENT polystyrene STANDARDS cover a MW range from 162 to 15 million g/mol, with MW STANDARDS selected to provide equidistant calibration points for greater informationPolystyrene individual molecular weightsPolymer Nominal Mp (g/mol)Nominal Mw/MnPart No , , , , , , , , , , , , , , , , , ,000, ,500, ,000, ,500, ,000, ,000, ,000, ,000, numbers are given for 1 g quantities.

10 (Part numbers for 5 g and 10 g quantities are obtained by replacing the last two digits, 01, with 05 or 10, respectively).ConditionsColumns: 2 x InfinityLab OligoPore, 7 5 x 300 mmEluent: THFFlow Rate: 1 0 mL/minDetector: PL-GPC 50 (DRI)Polystyrene standards10 POLYSTYRENE STANDARDSO rdering informationPolystyrene calibration kits, (all kits 10 x g)S-H-10 Part No PL2010-0103S-H2-10 Part No PL2010-0104 S-M-10 Part No PL2010-0100S-M2-10 Part No PL2010-0102S-L-10 Part No PL2010-0101S-L2-10 Part No PL2010-0105 Constituent POLYMER Nominal Mp (g/mol)300,0001,000580580162162 500,0003,0001,3001,300370370700,00010,00 05,0003,0005805801,000,00030,00010,0005, 0001,0001,0002,000,00070,00030,00010,000 2,0001,3003,000,000200,00070,00020,0003, 0002,0004,000,000700,000200,00030,0005,0 003,0007,000,0002,000,000500,00070,0007, 0005,00010,000,0004,000,0001,000,000130, 00013,0007,00015,000,00015,000,0003,000, 000300,00020,00010,00011 POLYMETHYLMETHACRYLATE STANDARDS Extreme versatility in solvent choice Solubility range across wide range of nonpolar and polar organic solvents First choice for calibration in polar organic solvents Certificate of analysis meets international protocolsPolymethylmethacrylate (PMMA) STANDARDS are available as a secondary standard option for organic solvents and as the preferred standard for polar organics such as DMSO, DMAc, DMF, and HFIP.