Agilent InfinityLab LC Series 1260 Infinity II ...

1 Agilent TechnologiesAgilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary PumpsUser ManualAgilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User ManualNotices Agilent Technologies, Inc. 2016 -2018No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla-tion into a foreign language) without prior agreement and written consent from Agi-lent Technologies, Inc. as governed by United States and international copyright Part NumberG7111-90000 Rev. CEdition08/2018 Printed in GermanyAgilent TechnologiesHewlett-Packard-Strasse 8 76337 WaldbronnWarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions. Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose.

2 Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor-mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such Rights LegendIf software is for use in the performance of a Government prime contract or sub-contract, Software is delivered and licensed as Commercial computer software as defined in DFAR (June 1995), or as a commercial item as defined in FAR (a) or as Restricted computer software as defined in FAR (June 1987) or any equivalent agency regu-lation or contract clause.

3 Use, duplication or disclosure of Software is subject to Agi-lent Technologies standard commercial license terms, and non-DOD Departments and Agencies of the Government will receive no greater than Restricted Rights as defined in FAR (c)(1-2) (June 1987). Government users will receive no greater than Limited Rights as defined in FAR (June 1987) or DFAR (b)(2) (November 1995), as applicable in any technical NoticesCAUTIONA CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated condi-tions are fully understood and met. WARNINGA WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death.

4 Do not proceed beyond a WARNING notice until the indi-cated conditions are fully understood and InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User Manual3In This This manual covers: Agilent 1260 Infinity II isocratic Pump (G7110B), Agilent 1260 Infinity II quaternary Pump VL (G7111A), Agilent 1260 Infinity II quaternary Pump (G7111B), and Agilent 1260 Infinity II Bio-inert Pump (G5654A).1 IntroductionThis chapter gives an introduction to the module, instrument overview and internal Site Requirements and SpecificationsThis chapter provides information on environmental requirements, physical and performance the PumpThis chapter provides information for optimized usage of the Optimizing PerformanceThis chapter gives hints on how to optimize the performance or use additional Troubleshooting and DiagnosticsThis chapter gives an overview about the troubleshooting and diagnostic features and the different user Error InformationThis chapter describes the meaning of error messages.

5 And provides information on probable causes and suggested actions how to recover from error InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User ManualIn This Test Functions and CalibrationThis chapter describes the tests for the MaintenanceThis chapter describes the maintenance of the for MaintenanceThis chapter provides information on parts for Identifying CablesThis chapter provides information on cables used with the Agilent 1200 Infinity Series Hardware InformationThis chapter describes the pump in more detail on hardware and LAN ConfigurationThis chapter provides information on connecting the module to the Agilent ChemStation AppendixThis chapter provides addition information on safety, legal and InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User Manual5 ContentsContents1 Introduction 9 Introduction to the Pump10G7110B isocratic Pump11G7111A quaternary Pump VL13G7111B quaternary Pump15G5654A Bio-inert Pump17 Overview of the Hydraulic Path19 Leak and Waste Handling272 Site Requirements and Specifications33 Site Requirements34 Physical Specifications37 Performance Specifications413 Using the Pump 49 Magnets50Tu r n o n / o f f51 Status Indicators53 Best Practices54 Leak and Waste Handling57 Hints for Successful Use of the Pump59 Setting up the Pump with the G4208A Instant Pilot60 Setting up the Pump with the Instrument Control Interface61 Solvent Information71 Algae Growth in HPLC Systems81 Prevent Blocking of Solvent Filters82 Normal Phase Applications834 Optimizing Performance85 Using the Degasser86 Operational Hints

6 For the Vacuum Degasser87 Operational Hints for the Multi Channel Gradient Valve (MCGV)88 When to use the Seal Wash Function89 Choosing the Right Pump Seals90 Optimize the Compressibility Compensation Setting916 Agilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User ManualContents5 Troubleshooting and Diagnostics93 User Interfaces94 Agilent Lab Advisor Software956 Error Information97 What Are Error Messages99 General Error Messages100 Module Error Messages1067 Test Functions and Calibration121 Introduction122 System Pressure Test123 Leak Rate Test1288 Maintenance135 Introduction to Maintenance and Repair136 Warnings and Cautions137 Overview of Maintenance and Repair138 Cleaning the Module139 Remove and Install Doors140 Checking and Replacing the Solvent Filter141 Exchange the Passive Inlet Valve142 Exchange the Outlet Valve144 Exchange the Purge Valve Frit or the Purge Valve146 Replace the O-Ring on the Purge Valve150

7 Remove the Pump Head Assembly153 Maintenance of a Pump Head Without Seal Wash Option155 Maintenance of a Pump Head with Seal Wash Option158 Reinstall the Pump Head Assembly162 Seal Wear-in Procedure165 Exchange the Multi-Channel Gradient Valve (MCGV)166 Replace the Solvent Selection Valve ( isocratic Pump)169 Exchange the Active Inlet Valve (AIV) or its Cartridge171 Exchange the Seal Wash Cartridge173 Replace Leak Handling System Parts174 Replacing the Module Firmware175 Agilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User Manual7 Contents9 Parts for Maintenance177 Accessory Kits178 Pump Head Assembly Without Seal Wash180 Pump Head Assembly with Seal Wash Option182 Outlet Valve185 Purge Valve Assembly186 Active Inlet Valve Assembly1881260 Infinity II Max Uptime Kit1901260 Infinity II Max Uptime Kit Bio1911260 Infinity II Low Dispersion Kit MCT192 HPLC System Tool Kit193 Solvent Cabinet194 Bottle Head Assembly195 Hydraulic Path of the quaternary Pump196 Hydraulic Path of the isocratic Pump19810 Identifying Cables201 Cable Overview202 Analog Cables204 Remote Cables206 CAN Cable210 Agilent Module to PC211 Agilent 1200 Module to Printer21211 Hardware Information213 Firmware

8 Description214 Electrical Connections217 Interfaces219 Setting the 6-bit Configuration Switch227 Instrument Layout231 Early Maintenance Feedback23212 LAN Configuration233 What You Have to Do First234 TCP/IP parameter configuration235 Configuration Switches236 Initialization Mode Selection237 Dynamic Host Configuration Protocol (DHCP)239 Manual Configuration2428 Agilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User ManualContents13 Appendix247 General Safety Information248 Waste Electrical and Electronic Equipment (WEE) Directive254 Radio Interference255 Sound Emission256 Agilent Technologies on Internet2579 Agilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User ManualAgilent Technologies1 IntroductionIntroduction to the Pump10G7110B isocratic Pump11 Product Description11 Features12G7111A quaternary Pump VL13 Product Description13 Features14G7111B quaternary Pump15 Product Description15 Features16G5654A Bio-inert Pump17 Product Description17 Features18 Overview of the Hydraulic Path19 Hydraulic Path20 How Does the Pump Work?

9 22 How Does Compressibility Compensation Work?25 How Does Variable Stroke Volume Work?26 Leak and Waste Handling27 Leak Sensor31 Waste Concept31 This chapter gives an introduction to the module, instrument overview and internal InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User Manual1 IntroductionIntroduction to the PumpIntroduction to the PumpThe Pumps comprise one pump head unit with an optional solvent cabinet, a vacuum degasser (optional for the isocratic pump), and a multi-channel gradient valve (MCGV) for quaternary pumps, or an optional solvent selection valve (SSV) for isocratic pumps. The combination of the pump head assembly and high speed proportioning MCGV with a possible connection up to four different solvents in quaternary pumps provides gradient generation by low pressure mixing. The combination of the pump head assembly and SSV in isocratic pump provides opportunity to make a choice between three different solvents.

10 A solvent cabinet provides enough space for eight one-liter bottles. The Active Seal Wash (ASW) and Active Inlet Valve (AIV) options are available for quaternary pumps when concentrated buffer solutions are used. The Bio-inert pump is optimized for these conditions, thus is always equipped with ASW and 1 Overview of the pump 'HJDVVHU 0&*9 ,QOHW 9 DOYH 2 XWOHW 9 DOYH 3 XPS +HDG 3 XUJH 9 DOYH Agilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User Manual11 Introduction1G7110B isocratic PumpG7110B isocratic PumpProduct DescriptionThe Agilent 1260 Infinity II isocratic Pump is ideal for demanding QA/QC tasks and routine applications. It maintains virtually pulse-free and stable solvent flows. The dual floating, precise servo-controlled pistons in the delivery mechanism adjust the stroke volume according to your chosen flow optional integrated degasser and solvent selection valve offers increased ease-of-use and method pump can deliver a broad range of pressures up to a maximum 600 bar, giving you the flexibility to use small particle size columns, longer columns or alternative higher viscosity Agilent 1260 Infinity II isocratic Pump is the ideal pump for GPC/SEC applications where run-to-run and day-to-day precision in retention times is 2 Overview of the pump 6 WDWXV /(' 3 XUJH YDOYH 3 RZHU VZLWFK /HDN GUDLQ 12 Agilent InfinityLab LC Series 1260 Infinity II isocratic - and quaternary Pumps User Manual1 IntroductionG7110B isocratic PumpFeatures Higher resolution and increased throughput with the new standard 600 bar pressure for modern LC columns.)