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Agilent InfinityLab LC Series 1260 Infinity II Binary Pump ...

Agilent InfinityLab LC Series1260 Infinity II Binary PumpUser ManualInfinityLab LC Series 1260 Infinity II Binary Pump User ManualInfinityLab LC Series 1260 Infinity II Binary Pump User ManualNoticesDocument InformationDocument No: SD-29000214 Rev. CEdition: 10/2021 Copyright Agilent Technologies, Inc. 2016-2021No part of this manual may be repro-duced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written con-sent from Agilent Technologies, Inc. as governed by United States and interna-tional copyright material contained in this document is provided as is, and is subject to being changed, without notice, in future edi-tions.

1260 Infinity II Binary Pump offers reproducible gradients and high-performance, providing high-throughput and fast separati ons. The pump is suitable for routine applications, with UV or MS detection, where high speed and resolution with uncompromised data quality are required. Figure 4 Overview of the pump Features

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Transcription of Agilent InfinityLab LC Series 1260 Infinity II Binary Pump ...

1 Agilent InfinityLab LC Series1260 Infinity II Binary PumpUser ManualInfinityLab LC Series 1260 Infinity II Binary Pump User ManualInfinityLab LC Series 1260 Infinity II Binary Pump User ManualNoticesDocument InformationDocument No: SD-29000214 Rev. CEdition: 10/2021 Copyright Agilent Technologies, Inc. 2016-2021No part of this manual may be repro-duced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written con-sent from Agilent Technologies, Inc. as governed by United States and interna-tional copyright material contained in this document is provided as is, and is subject to being changed, without notice, in future edi-tions.

2 Further, to the maximum extent permitted by applicable law, Agilent dis-claims all warranties, either express or implied, with regard to this manual and any information contained herein, includ-ing but not limited to the implied warran-ties of merchantability and fitness for a particular purpose. Agilent shall not be lia-ble for errors or for incidental or conse-quential damages in connection with the furnishing, use, or performance of this document or of any information con-tained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such Rights Government Restricted Rights.

3 Soft-ware and technical data rights granted to the federal government include only those rights customarily provided to end user customers. Agilent provides this customary commercial license in Soft-ware and technical data pursuant to FAR (Technical Data) and (Computer Software) and, for the Depart-ment of Defense, DFARS (Technical Data - Commercial Items) and DFARS (Rights in Commer-cial Computer Software or Computer Software Documentation).Safety NoticesCAUTIONA CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met.

4 WARNINGA WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in personal injury or death. Do not proceed beyond a WARN-ING notice until the indicated conditions are fully understood and Technologies Hewlett-Packard-Strasse 8 76337 Waldbronn, GermanyInfinityLab LC Series 1260 Infinity II Binary Pump User Manual3In This manual covers the Agilent InfinityLab LC Series 1260 Infinity II Binary Pump (G7112B).1 IntroductionThis chapter gives an introduction to the module and an instrument Requirements and SpecificationsThis chapter provides information about site requirements and specifications for the Binary the PumpThis chapter explains the operational parameters of the Binary PerformanceThis chapter gives information on how to optimize the performance of the Binary Pump under special operational and DiagnosticsOverview of the troubleshooting and diagnostic InformationThis chapter describes the meaning of error messages.

5 And provides information on probable causes and suggested actions how to recover from error Functions and CalibrationThis chapter explains all test functions that are available for the Binary chapter describes the maintenance of the LC Series 1260 Infinity II Binary Pump User Manual49 Parts and Materials for MaintenanceThis chapter lists all parts and tools that are required for maintenance and simple CablesThis chapter provides information on cables used with the Agilent InfinityLab LC Series InformationThis chapter provides detailed technical information about your Binary ConfigurationThis chapter provides information on connecting the module to the Agilent ChemStation chapter provides addition information on safety.

6 Legal and LC Series 1260 Infinity II Binary Pump User Manual5 Contents1 Introduction10 Instrument and Operation11 Overview of the Hydraulic Path15 Leak and Waste Handling202 Site Requirements and Specifications25 Site Requirements26 Physical Specifications29 Performance Specifications303 Using the Pump32 Magnets33 Turn on/off34 Status Indicators36 Best Practices37 Leak and Waste Handling39 Hints for Successful Use of the Binary Pump40 Setting up the Pump with the G4208A Instant Pilot41 Setting up the Pump with the Instrument Control Interface42 Solvent Information47 Algae Growth in HPLC Systems54 Prevent Blocking of Solvent Filters55 Normal Phase Applications574 Optimizing Performance59 When to Use a Vacuum Degasser60 When to Use the Active Seal Wash Option61 When to Use the Low Volume Mixer62 When to Remove Damper and Mixer63 How to Optimize the Compressibility Compensation Setting65 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual65 Troubleshooting and Diagnostics68 Overview of the Module s Indicators and Test Functions69 User Interfaces71 Agilent Lab Advisor Software726 Error Information73 What Are Error Messages74 General Error Messages75 Module Error Messages817 Test Functions and Calibration92(System)

7 Pressure Test93 Valve Test98 Binary Pump Solvent Compressibility Calibration100 Pump Elasticity Calibration102 Pump Leak Rate Test104 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual78 Maintenance108 Introduction to Maintenance109 Cautions and Warnings110 Cleaning the Module112 Overview of Maintenance and Simple Repairs113 Maintenance Procedures114 Remove and Install Doors115 Exchange the Purge Valve Frit or the Purge Valve117 Replace the O-Ring on the Purge Valve120 Remove the Pump Head Assembly122 Maintenance of a Pump Head without Seal Wash124 Maintenance of a Pump Head with Seal Wash127 Reinstall the Pump Head Assembly131 Seal Wear-in Procedure133 Exchange the Active Inlet Valve (AIV) or its Cartridge134 Exchange the Seal Wash Cartridge137 Replace Leak Handling System Parts139 Exchange the Outlet Valve141 Installation of the Solvent Selection Valve Upgrade Kit143 Exchange the Solvent Selection Valve145 Replacing Module Firmware148 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual89 Parts and Materials for Maintenance149 Hydraulic Path with Solvent Selection Valve150 Hydraulic Path without Solvent Selection Valve152 Pump Head Assembly Without Seal Wash154 Pump Head Assembly with Seal Wash Option156 Outlet Valve158 Purge Valve Assembly159 Active Inlet Valve Assembly160 HPLC System Tool Kit161 Active Seal Wash Option162 Solvent Cabinet163 Bottle Head Assembly164 Accessory Kit165 Cover

8 Parts16610 Identifying Cables167 Cable Overview168 Analog Cables170 Remote Cables172 CAN/LAN Cables176RS-232 Cable Kit177 Agilent 1200 Module to Printer17811 Hardware Information179 Firmware Description180 Electrical Connections183 Interfaces185 Setting the 6-bit Configuration Switch193 Early Maintenance Feedback197 Instrument Layout198 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual912 LAN Configuration199 What You Have to Do First200 TCP/IP parameter configuration201 Configuration Switches202 Initialization Mode Selection203 Dynamic Host Configuration Protocol (DHCP)205 Manual Configuration20813 Appendix212 General Safety Information213 Waste Electrical and Electronic Equipment (WEEE) Directive219 Radio Interference220 Sound Emission221 Agilent Technologies on the Internet222 InfinityLab LC Series 1260 Infinity II Binary Pump User Manual101 IntroductionInstrument and Operation11 Introduction to the Pump11 Principle of Operation12 Product Description14 Features14 Overview of the Hydraulic Path15 Leak and Waste Handling20 Leak Sensor23 Waste Concept24 This chapter gives an introduction to the module and an instrument LC Series 1260 Infinity II Binary Pump User Manual111 IntroductionInstrument and OperationInstrument and OperationIntroduction to the PumpThe Binary pump comprises two identical pumps integrated into one housing.

9 Binary gradients are created by high-pressure mixing. A built-in degasser is available for applications that require best flow stability, especially at low flow rates, for maximum detector sensitivity. Pulse damper and mixer can be bypassed for low flowrate applications or whenever a minimal transient volume is desirable. Typical applications are high throughput methods with fast gradients on high resolution mm columns. The pump is capable of delivering flow in the range of 5 mL/min against up to 600 bar. A solvent selection valve (optional) allows to form Binary mixtures (isocratic or gradient) from one of two solvents per channel. Active seal wash (optional) is available for use with concentrated buffer 1 Overview of the Binary pump1 IntroductionInstrument and OperationInfinityLab LC Series 1260 Infinity II Binary Pump User Manual12 Principle of OperationThe Binary pump is based on a two-channel, dual-piston in- Series design which comprises all essential functions that a solvent delivery system has to fulfill.

10 Metering of solvent and delivery to the high-pressure side are performed by two pump assemblies which can generate pressure up to 600 channel comprises a pump assembly including pump drive, pump head, active inlet valve with replaceable cartridge, and outlet valve. The two channels are fed into a low-volume mixing chamber which is connected via a restriction capillary coil to a damping unit and a mixer. A pressure sensor monitors the pump pressure. A purge valve with integrated PTFE frit is fitted to the pump outlet for convenient priming of the pumping 2 The hydraulic path of the Binary Pump with damper and mixer SSV Seal wash pump Pump head A Pump head B Damper Mixer Pressure sensor Mixing chamber Inlet valve Inlet valve Outlet valve Outlet valve to waste to sampler Purge valve InfinityLab LC Series 1260 Infinity II Binary Pump User Manual131 IntroductionInstrument and OperationDamper and mixer can be bypassed for lowest delay volume of the Binary pump.


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