Transcription of Agilent SureCycler 8800
1 Agilent SureCycler 8800 CHOOSING THE RIGHT THERMAL CYCLERHAS NEVER BEEN EASIERFUNCTIONALITY FOR YOUR LABSURELY BETTER PCREasy to learn moreSee for yourself why the SureCycler 8800 is a Surely Better solution for lab managers and researchers. Visit to view a short video of the all-in-one features and accessPCR WizardPre-loaded protocolsSuperior customer support 8VH D ZHE HQDEOHG GHYLFH WR PRQLWRU \RXU experiment from across the room or across town 1 HWZRUNLQJ IHDWXUHV LQFOXGHG QR H[WUD VRIWZDUH RU hardware purchase required. 6 DYH WLPH XVLQJ $JLOHQW V PRVW SRSXODU HQ]\PH protocols pre-loaded on the SureCycler 8800 (DVLO\ DGG QHZ SURWRFROV RU FKRRVH IURP D OLVW RI $JLOHQW GHYHORSHG SURWRFROV ,PSURYH \RXU UHVHDUFK DQG UHGXFH VWDUW XS WLPH ZLWK RXU LQWXLWLYH SURWRFRO ZL]DUG 4 XLFNO\ FUHDWH QHZ SURWRFROV WDLORUHG WR \RXU research in 5 steps1RZ \RX FDQ FDOO RQH WUXVWHG SDUWQHU WR JHW KHOS LQ GHVLJQLQJ \RXU DVVD\V RSWLPL]LQJ \RXU SURWRFROV DQG WURXEOHVKRRWLQJ \RXU SURJUDPV $JLOHQW V H[SHULHQFHG technical support team is available to help with your complete portfolio questions.)
2 0D\ QRW ZRUN ZLWK VRPH GHYLFHVThe Agilent SureCycler 8800 is one easy decision to a complete portfolio of PCR solutions SURELY BETTER PCREasy interchangable moduleGradient enables single run optimization of your protocolThe easy choice for both speed and volume +LJKHVW VSHHG DQG H[LEOH reaction volumes $JLOHQW V HQ]\PHV ZLWK )XVLRQ technology perform with the speed of the instrument to produce faster and better resultsThe easy choice for flexibility with 96 and 384 well blocks 4 XLFNO\ FRQYHUW LQWHUFKDQJHDEOH EORFNV EDVHG RQ \RXU ODE V QHHGVThe easy choice for superior uniformity and gradient *UDGLHQW IXQFWLRQDOLW\ ZLWKRXW VDFUL FLQJ XQLIRUPLW\ ([FHOOHQW JUDGLHQW IXQFWLRQDOLW\ IRU DFFHOHUDWHG protocol development and unique applicationsThe easy choice for simple interface and established functionality KLJK UHVROXWLRQ WRXFK VFUHHQ ZLWK LQWXLWLYH easy-to-use software ,QWXLWLYH.]
3 L]DUGV FUHDWH SURWRFROV GHVLJQHG IRU \RXU ODE V FRQGLWLRQVM arket leading uniformity gives superior results every time you run your protocol6 C/secMarket-leading cycling speed and volumes from 10 to 100 lSURELY BETTER EnzymesThe right enzyme for every applicationIT S EASY TO BE CONFIDENT ABOUT THE +HUFXODVH ,, )XVLRQ '1$ 3RO\PHUDVH 3 URYLGHV WKH KLJKHVW \LHOGV DFURVV WKH EURDGHVW UDQJH of targetsPfuUltra ,, +RWVWDUW '1$ 3RO\PHUDVH DQG 0 DVWHU 0L[ 7KH ZRUOG V PRVW DFFXUDWH SRO\PHUDVHHigh Fidelity Fusion PCR EnzymesRoutine PCR EnzymesPaq5000 '1$ 3RO\PHUDVH DQG 0 DVWHU 0L[ )]
4 XVLRQ WHFKQRORJ\ DOORZV IRU ORQJHU WDUJHWV DQG IDVWHU results for routine PCR Taq2000 and SureStart Taq '1$ 3RO\PHUDVHVPaq5000 HotstartPCR Master MixClosestCompetitor6 kbAmplification of , , and kb fragments of Human a-1 Antitrypsin gene, and 6 kb fragment of human -gene amplified using Paq5000 Hotstart PCR Master Mix and the closest hot start Taq master mix Enzymes$FFX6 FULSW Reverse Transcriptase 7KH ZRUOG V PRVW DFFXUDWH UHYHUVH WUDQVFULSWDVH$I QLW\6 FULSW Reverse Transcriptase 6 HQVLWLYLW\ FRPELQHG ZLWK VXSHULRU \LHOGV DFURVV D broad temperature rangeSpecialty PCR Enzymes(DV\ $ +LJK )
5 LGHOLW\ Polymerase and Master Mix +LJK GHOLW\ $1' $ RYHUKDQJV IRU 7$ 8$ FORQLQJPicoMaxx '1$ 3RO\PHUDVH DQG 0 DVWHU 0L[ 7KH PRVW VHQVLWLYH SRO\PHUDVH DYDLODEOH IRU UDUH '1$PfuTurbo Cx Hotstart '1$ 3RO\PHUDVH 7KH RQO\ SRO\PHUDVH WR FRPELQH KLJK GHOLW\ and the DELOLW\ WR UHDG WKURXJK G873 SUHVHQW LQ WKH DPSOL FDWLRQ ELVXO WH VHTXHQFLQJ '1$ GDPDJH HWF Herculase II Fusion DNA Polymerase produced superior yields of 6 kb human genomic DNA fragment at extension rates of 15-60 sec/kb. The experiment was conducted using each enzyme s recommended buffer and unit concentration.]
6 +HUFXODVH ,, (Q]\PHClosest competitorENTIRE PORTFOLIORT-PCR Kits(DV\ $ 2QH 7 XEH RT-PCR System 7KH RQO\ RQH VWHS 57 3&5 V\VWHP RIIHULQJ ERWK 7$ 8$ FORQLQJ DQG KLJK GHOLW\$FFX6 FULSW 3IX8 OWUD ,, 57 3&5 .LW +LJKHVW GHOLW\ 57 FRPELQHG ZLWK KLJKHVW GHOLW\ 3&5 HQ]\PH RQ WKH PDUNHW$I QLW\6 FULSW 2QH 6 WHS 57 3&5 .LW 'HOLYHUV WKH KLJKHVW \LHOG DQG VHQVLWLYLW\ RI DQ\ RQH VWHS 57 3&5 NLWSite-Directed Mutagenesis Kits2XU VLJQDWXUH WKUHH VWHS 4 XLN&KDQJH PHWKRG XWLOL]HV OLQHDU DPSOL FDWLRQ GXULQJ PXWDJHQHVLV WR VLJQL FDQWO\ GHFUHDVH unwanted second-site /LJKWQLQJ 6 LWH 'LUHFWHG 0 XWDJHQHVLV.))
7 LW 3 HUIRUP PXWDJHQHVLV WZLFH DV IDVW DV RULJLQDO 4 XLN&KDQJH DQG 4 XLN&KDQJH ,, 8VH RQH NLW IRU ERWK VKRUW DQG ORQJ WHPSODWHV NE 4 XLN&KDQJH /LJKWQLQJ Multi 6 LWH 'LUHFWHG 0 XWDJHQHVLV .LW 0 XWDWH XS WR VLWHV VLPXOWDQHRXVO\ WLPHV IDVWHU WKDQ RXU RULJLQDO NLW/HDUQ PRUH DERXW WKH $JLOHQW 6 XUH&\FOHU DW BETTER Reagent KitsNow it s easy to be sure about getting good resultsPicoMaxxHigh-Fidelity PCR SystemClosestCompetitor AClosestCompetitor BGenomic DNAT emplate Qty.(ng) kb502510150251015025101 Superior sensitivity with PicoMaxx High-Fidelity PCR System. Amplification of a kb a-1 anti-trypsin template compared to Agilent s two closest competitors.
8 All reactions were performed according to the manufacturer s sensitivity with the AffinityScript One-Step RT-PCR Kit. Total RNA at varying concentrations (1, 10 and 100 ng) was amplified for multiple targets, M1: 1 kb DNA Ladder; M2: lambda/HindIII marker060120204 QuikChange LightningThermal CyclingQuikChangeThermal CyclingDigestionDigestion601386051326048 55 Time (min)180240300360128 Template size (kb)427660 QuikChange Lightning saves hours of valuable research time. Three template sizes were mutated with both QuikChange and QuikChange Lightning Mutagenesis Kits.
9 Times were measured for both thermal cycling and block384-well block0D[LPXP %ORFN 5 DPS 5 DWH6 C/sec 4 C/sec%ORFN 7 HPSHUDWXUH 5 DQJH4 C to 99 C 4 C to 99 C7 HPSHUDWXUH $FFXUDF\ (@95 C) (@95 C)Temperature Uniformity (@95 C) (@95 C)GradientYes YesGradient Temperature Range 30 C to 99 C 30 C to 99 CMaximum Gradient30 C 30 CVolume Capacity10-100 l 5-25 lThis information is subject to change without notice. Agilent Technologies, Inc. 2012, 2016 Printed in the USA, February 4, 2016 5990-7749 ENPR7000-0381 For more information Learn more or buy online an Agilent customer center in your country and Canada 1-800-227-9770 Pacific Agilent SureCycler 8800 gives you the best overall specification packageMemory Capacity SURWRFROVPre-loaded ProtocolsYes3&5 :L]DUGYe s'LVSOD\ 6L]H7 inchesDisplay Resolution800 x 480 Display TypeFull Touch ScreenPorts2 x USB L x 28 W x 27 H (38 open) cmWeight NJStandard Warranty2 year800 StrataClonePCR Optimization and Purification 3&5 3 ROLVKLQJ.
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