Ammonium Sulfate Solution (A5479) - Bulletin
Ammonium sulfate is widely used for precipitation and fractionation of proteins 1 as well as for the crystallization of proteins 2,3,4 and protein-nucleic acid complexes.5 Ammonium sulfate is also utilized in hydrophobic interaction chromatography6,7,8 and antibody purification.9,10 Ammonium sulfate acts by pulling water molecules
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document:
Documents from same domain
Guide to Solid Phase Extraction - Sigma-Aldrich
www.sigmaaldrich.comSUPELCO 2 Bulletin 910 SPE tubes that are packed with this material contain stainless steel or Teflon ® frits, required by US Environmental …
Guide, Phases, Sigma, Environmental, Aldrich, Sigma aldrich, Solid, Extraction, Guide to solid phase extraction
Instructions for Chiral-AGP, Chiral-HSA and Chiral …
www.sigmaaldrich.comThis Data Sheet Contains Important Information About This Product. Operating Guidelines for ChromTech CHIRAL-AGP, CHIRAL-HSA, and CHIRAL-CBH HPLC Columns
Hplc, Columns, Chiral, Chiral agp, Chiral hsa and, Chiral hsa, And chiral cbh hplc columns
Sunstone Upconverting Nanocrystals UCP 475, Carboxylated ...
www.sigmaaldrich.comSunstone Upconverting Nanocrystals UCP 475, Carboxylated Catalog Number 90838 Storage Temperature 2–8 °C TECHNICAL BULLETIN Product Description Sunstone Upconverting Nanocrystals (UCP) are a novel and proprietary class of rare earth doped
Carboxylated, Sunstone, Sunstone upconverting nanocrystals ucp 475, Upconverting, Nanocrystals
GC Column Selection Guide - Sigma-Aldrich
www.sigmaaldrich.comThis is the single most important characteristic in selecting a capillary column because it dictates selectivity, or the ability of the column to separate sample components. Phase selection is based on the general chemical principle that “likes dissolves like.” A non-polar column is best for the analyses of non-polar compounds. Polar
Urea Solution (U4883) - Datasheet - Sigma-Aldrich
www.sigmaaldrich.comDilution Table – These dilutions are based on a 25 ml starting volume of 8 M Urea Solution. Desired Molarity Volume of water to add to 25 ml of prepared 8 M Urea Solution Final Volume 8 M 0.0 ml 25.0 ml 7 M 3.6 ml 28.6 ml 6 M 8.3 ml 33.3 ml 5 M 15.0 ml 40.0 ml 4 M 25.0 ml 50.0 ml
Greener Solvent Alternatives - Sigma-Aldrich
www.sigmaaldrich.comFor more information, visit sigma-aldrich.com 3 CPME is a proven alternative to THF, providing better yields and higher selectivity. OCH 3 Cyclopentyl methyl ether (CPME)
QUANTITATIVE NMR - Sigma-Aldrich
www.sigmaaldrich.comFeb 08, 2017 · water, residual solvents and inorganic impurities) and calculating the content by subtracting the impurity values from a total of 100%. This method however implies that no potential impurities ... available from Sigma-Aldrich, which we use to ... a question of what level of precision should finally be achieved.
RIPA Buffer (R0278) - Technical Bulletin
www.sigmaaldrich.comRIPA Buffer is supplied as a ready to use solution that requires no preparation. Protease and phosphatase inhibitors may be added to the lysis buffer as needed. One ml of the RIPA Buffer is sufficient to lyse cells from one 100 mm culture dish (0.5 to 5 107 cells) of most adherent mammalian cell lines. Components
Related documents
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins might denature, making the …
Nutrients Plants Require for Growth - uidaho.edu
www.extension.uidaho.eduplant root zones with precipitation or irrigation. A typical plant contains 0.1 percent S on a dry weight basis, but this can range from 0.05 to 0.5 percent S. Sulfur, like N, is a component of some amino acids that link together to form proteins. Sulfur is also a component of plant protoplasts and enzymes (table 2). Once in the plant, sulfur ...
Plasmid Isolation (Alkaline Lysis) - G-Biosciences
www.gbiosciences.comprecipitation of a SDS-protein complex as a white precipitate, consisting of SDS, lipids and proteins. In addition, the pota ssium acetate neutralizes the solution allowing the renaturation of the DNA. The large chromosomal DNA is captured in the precipitate, whereas the small plasmid DNA remains in solution. Page 7 of 12
Isolation, Alkaline, Protein, Precipitation, Plasmid, Lysis, Plasmid isolation, Alkaline lysis
TRIzol Reagent Product description - Thermo Fisher Scientific
assets.thermofisher.comTRIzol™ Reagent allows to perform sequential precipitation of RNA, DNA, and proteins from a single sample (Chomczynski, 1993). After homogenizing the sample with TRIzol™ Reagent, chloroform is added, and the homogenate is allowed to separate into a clear upper aqueous layer (containing RNA), an interphase, and a red lower organic layer
Whey Protein Concentrate - Agricultural Marketing Service
www.ams.usda.govWhey is the soluble fraction of milk, rich in proteins, minerals and lactose that are separated from casein 107 during the manufacture of cheese or casein (Table 1). This separation is usually accomplished by ... precipitation of the caseins forming curd (Foegeding et al., 2011). 117 .
Services, Agricultural, Marketing, Protein, Precipitation, Agricultural marketing service
00-305/1156 acryl.poly tn - Bio-Rad Laboratories
www.bio-rad.comcause artifacts such as aberrant relative mobility, precipitation of some proteins and nucleic acids, streaking or smearing of bands, and run-to-run irreproducibility. In acrylamide, acrylic acid should be below 0.001% (w/w). This is determined by direct titration, and supported by both conductivity and pH measurement. 2.
RapiGest SF Surfactant - Waters Corporation
www.waters.comHYDROPHOBIC PROTEINS VII. SUGGESTED SAMPLE PREPARATION FOR LC AND LCMS VIII. SUGGESTED SAMPLE PREPARATION FOR GLYCOPROTEIN SAMPLES FOR RAPID DEGLYCOSYLATION RapiGest SF Surfactant I. INTRODUCTION RapiGest™ SF is a reagent used to enhance in-gel 1 and in-solution 2-6 enzymatic digestions of proteins. Recent …
A Rapid and Sensitive Method for the Quantitation of ...
hoffman.cm.utexas.eduprocedure, too, has its drawbacks since the precipitation of the protein by trichloroacetic acid followed by filtration on Millipore membranes is required. The protein assay herein described eliminates most of the problems involved in the procedures described above, and …
Fundamentals of Protein Chemistry - Duke University
genome.duke.eduProteins may be run directly, or are denatured, reduced, and alkylated. Samples, with a visible dye added, are loaded in wells cut into the top of the gel. Loading capacity depends on gel size and thickness. In 2D IEF/PAGE, the gel strip from IEF is loaded into a single large well. Molecular weight standards are often run to calibrate the gel.