初心者のための 入門 - Beckman Coulter

1 FCMFCM FCMFCM .. FFlowlowCCytoytoMMetryetry FFlowlowCCytoytoMMetereter 1 / 100 / 11

2 DNA FCMFCM 5050 mm 11 55 101066 / mL/ mL 200200 LL 11 11 FCMFCM

3 ArAr (488nm)(488nm)HeHe--NeNe (633nm(633nm (405nm)(405nm)ArAr UVUV(355nm)(355nm) 500LP600 DLP615 DSP710 DLP550 DLP620SP675BP755BP575BP525BP488 DLPSS488nm 635nm FSFL1FL2FL3FL4FL5 Cytomics FC 500 Cytomics FC 500 FSFS SSSS (SS) (FS) 1.))

4 1. zz zz Cytomics FC 500 Cytomics FC 500 PC7755FL5 APC/C 57-AADPC5675FL4 PIECD620FL3PE/RD1575FL2 FITC525FL1 DNA Ar --22 FSFS SSSS GRANULOCYTE FS SS

5 FCM FCM SSFS Double NegativeSingle PositiveSingle PositiveDouble Positive 22 LyLyLyLy LyLyMoMoGrGrLyLyLy 22 55.. --22 -- -- -- -- -- A/D - - FSFS 1010

6 FSFS 150150 FSFS 250250 FS SS 11 Mean Y Mean X FL2FL2--%FL1%FL1FL1FL1--%FL2%FL2 FITCFITC FL1 LogFL1 Log XX PEPE FL2 LogFL2 Log YY 1 FITC FL2 2 PE FL1 22 33 ADCADC Double NegativeSingle PositiveSingle PositiveDouble Positive22 FCMFCM A/D

7 Two-Color CD34 Allergenicity 1001,00010,000100, 3002,50011,00044,00029,00080 Molecule And Receptor QuantitationUsing ImmunofluorescenceStandardization Web