BIOSTAT CultiBag RM Culturing Convenience - …

1 BIOSTAT CultiBag RM. Culturing Convenience turning science into solutions Culturing Convenience The BIOSTAT CultiBag RM is The measurement and control Technical Specifications the new generation of dispos- capabilities supplied by able bioreactors utilizing rock- Sartorius Stedim Biotech are Dimensions Weight (kg). ing motion for mixing with second to none. Utilizing W + H + D (mm). low shear. The combination of proven technology and expert BIOSTAT Basic housing 320 + 735 + 565 mm 50 kg a new generation of Sartorius engineering, we have developed Stedim Biotech engineered our existing in-house systems Rocker 2 with Holder 2 330 + 400 + 430 mm 7 kg control capabilities launches to bring powerful control Rocker 20/50 with the disposable bioreactor sys- capabilities to the disposable Holder 20 710 + 400 + 560 mm 27 kg tem into a new era of cultiva- market.

2 The BIOSTAT Control tion. The CultiBag RM is special- system presents an easy-to- Rocker 20/50 with ly designed for shear sensitive use touch screen control sys- Holder 50 1030 + 450 + 580 mm 32 kg cells and any single use applica- tem with integrated measure- Rocker 200 2075 + 1038 + 1266 mm 230 kg tions. A comprehensive valida- ment and control hardware, tion and extractables report is pumps, temperature and Utilities available for the bags and also gassing systems, for excellent Power supply 120 VAC and 230 VAC. can be designed for your process control.

3 208 VAC and 400 VAC. process. The application-driven, config- Maintenance and overhead ured packages for basic, optical expenses are reduced, eliminat- and perfusion provide every- ing the need for: thing needed to get started immediately. The BIOSTAT . Sterilization in Place (SIP). CultiBag RM is available with Clean In Place (CIP) scalable working volumes from L to 100 L. Just select the Reduction in WFI. size that meets your needs Reduction in user time | today. Each basic, optical and effort perfusion package also includes our MFCS/DA software package Reduction in validation for data collection and manipu- requirements lation.

4 Exportable into Excel this is a powerful tool which can be upgraded to multi-level MFCS/win control system. The BIOSTAT CultiBag RM. is ideal for Cell culture, growth of ani- mal, insect and plant cells Shear sensitive cells or anaerobic bacteria Transition from shake or tissue culture flasks Cultivation of Seed for Traditional Bioreactors Production scale up to 100 L. For all your disposable applications Packages The BIOSTAT CultiBag RM. packages are specially config- ured to meet your disposable needs: Basic Packages Basic Packages are used for simple and easy cultivation.

5 The rocker is used as a standalone unit with the heater blanket for temperature control and is equipped with an integrated air pump and flow controller Basic Packages Optical Packages Perfusion Packages for bag inflation and supply to Standalone Unit Standalone Unit Standalone Unit cells. Alternatively the unit may be placed inside an incubator Rocker only Rocker with Optical Tower Rocker with Perfusion Tower for temperature control and Integrated pump for inflation incubator air is supplied to Optical Tower Perfusion Tower of bag and supply of ambient the cells.

6 Additionally pressure air to the cells Control of disposable optical Balances for feed |harvest relief and flow controller is chemical pH and DO sensors flow control are included included for attachment to Pressure relief and flow con- are included your own gas mixing device. troller for connection to an Control of disposable optical Rocking rate and rocking angle external gas supply device Feedback control of pH chemical pH and DO sensors can be optimized. are included Rocking angle and rocking Feedback control of DO. rate control Feedback control of pH.

7 Optical Packages Intuitive touchscreen Optical Packages are designed Temperature control heater Feedback control of DO. Trend display for batch and fed-batch blanket included Intuitive touchscreen processes, with the control of 2 integrated pumps Rocker may alternatively be disposable optical chemical pH Trend display placed into a CO2 incubator Pressure control for and DO sensors. Feedback con- overpressure safety 4 integrated pumps trol of both dissolved oxygen MFCS/DA data logging by rocking rate and pH control software included Gassing by 4 + rotameters Pressure control for is available for your process.

8 And 2 + mass flow controllers overpressure safety Additionally two integrated to overlay aeration Gassing by 4 + rotameters pumps are supplied inclusive of MFCS/DA data logging and 2 + mass flow controllers dosing counters. MFCS/DA data software included to overlay aeration logging software is included. MFCS/DA data logging Perfusion Packages software included Perfusion Packages are Bag Designs designed for perfusion control, in addition to Optical Controller Basic Bags* Optical Bags* Perfusion Bags*. capabilities. Four integrated pumps are supplied for feed | Air inlet, air outlet Air inlet, air outlet Air inlet, air outlet harvest control by weight.

9 Sample port Sample port Sample port Balances are included for excellent perfusion control. Spare port with female luer Spare port with female luer Spare port with female MFCS/DA data logging connector connector luer connector software is included. 2 + Additional fittings 2 + Additional fittings 2 + Additional fittings Fill|harvest fittings Optical chemical DO sensor Optical chemical DO sensor Optical chemical pH sensor Optical chemical pH sensor Fill |harvest fittings Internal fixed perfusion membrane (pore size m;. other pore sizes will be Customised bags also available available soon).

10 * Bags not included in packages Fill |harvest fittings Bag Technical Specifications Description Standard Bags Cat. No. CultiBag Basic DBB001L2 DBB002L DBB010L DBB020L DBB050L DBB100L DBB200L. DBB002L2. CultiBag Optical DBO002L DB010L DBO020L DBO050L DBO100L DBO200L. DBO002L2. CultiBag Perfusion DBP002L DBP020L. Total Volume (L) 1 2 10 20 50 100 200. Min Working Volume (L) * * 1* 2* 5* 10* 20*. Max Working Volume (L) 1 5 10 25 50 100. Rocker Types Rocker 2 with Holder 2 . Rocker 20/50. with Holder 20 . Rocker 20/50. with Holder 50.