Transcription of Cell Counting Kit-8 Technical Manual
1 - 100 : - 500 :- 2500 :- 10000 :- (450 nm ) - 96 - CO2 - 10 l 100 - 200 l Cell Counting Kit-8 (CCK-8) WST-8( 1) WST-8 460 nm ( 2) CCK-8 1 / NADH 1-Methoxy PMS WST-8 ( 2) CCK-8 MTT [3H] - (96 ) 1. 5000 cells/well 96 100 l CO2 , 2. 10 l CO2 (6, 12 , 24,48 ) , * 10 l * 1 - 4 * 5.
2 450 nm * 10 l * 24 * mol/l HCl 1 w/v% SDS 1 WST-8 CK04: Cell Counting Kit-8 Revised August 26, 2016 100 l 37 C CO2 10 l 37 C CO2 CCK-8 10 l 37 C CO2 1-4 450 nm HL60 CellNumber of Cells (x103cells/well)HeLa CellAbsorbanceNumber of Cells (x103cells/well) 2 CCK-8 4 C 1 -20 C 3 Medium : HeLa: MEM, 10% FBSHL60: RPMI1640, 10% FBSI ncubation : HeLa: 37oC, 5% CO2, 2 hoursHL60: 37oC, 5% CO2, 3 hoursDetection : 450 nmAbsorbanceCell Counting Kit-8 Technical Manual - / -100 , 500 2500 , 10000 1 ml 15 ml 15 ml 5100 ml 1 1.
3 M. Ishiyama, Y. Miyazono, K. Sasamoto, Y. Ohkura and K. Ueno, Talanta, 1997, 44, H. Tominaga, M. Ishiyama, F. Ohseto, K. Sasamoto, T. Hamamoto, K. Suzuki and M. Watanabe, Anal. Commun.,1999, 36, 96 1 1000 cells 2500 cells 1 96 25000 cells 24 6 96 4 16 1/10 CCK-8 2. CCK-8 WST-8 WST-8 3. 4. CCK-8 5.
4 450 nm 430 - 490 nm 450 nm FAQ1. 2. WST-8 3. 4. m 5. 6. 600 - 650 nm 1 Cell line : CTLL-2 Culture medium : RPMI1640, 10% FBSDrug :Human Interleukin-2 Exposure : 72 hoursIncubation : 37 C, 5% CO2, 4 hoursDetection : 450 nmCell line : HeLaCulture medium : DMEM, 10% FBSDrug : Actinomycin DExposure : 24 hoursIncubation : 37 C, 5% CO2, 4 hoursDetection : 450 nmConcentration of IL-2 (ng/ml)Concentration Actinomycin D ( g/ml)Absorbance at 450 nmAbsorbance at 450 nm 4 Actinomycin D) 5 IL-2) 1 10 100 2025-5 861-2202 Tel:096-286-1515 ( ) Fax:096-286-1525 E-mail: URL.
5 ( ) 2-1-17 7F 105-0012 Tel: 03-3578-9651 ( ) Fax: 03-3578-9650 :0120-489548 :0120-021557CK04 : Cell Counting LDH assay Kit-WSTAbs 490 nmCell Counting Kit-8 Abs 450 nmMitomycin C ( mol/l)Cell Counting Kit-8 Cytotoxicity LDH Assay Kit-WST 6 Cell Counting Kit-8 Cytotoxicity LDH Assay Kit-WST [Code: CK12] Cell line : HeLaCulture medium : MEM, 10% FBSDrug : Mitomycin CExposure : 37 C, 5% CO2, 48 hoursDetection : Cell Counting Kit-8 (450 nm) Cytotoxicity LDH Assay Kit-WST (490 nm)Cell Counting Kit-8 (Absorbance: 450 nm)Cytotoxicity LDH Assay Kit-WST (Absorbance: 490 nm)
