Example: bankruptcy
Chromatography Band-Broadening (rate theory)
B. Theoretical measures of band-broadening: 1. In chromatographic theory, the width of a peak is usually described in terms of H, the high equivalent of a theoretical plate, H = L/n L = colume length N= number of theoretical plates = (tR/σ)2 tR= retention time of the solute σ=standard deviation of the peak (in time units)
Tags:
Information
Domain:
Source:
Link to this page:
Documents from same domain
Diffusion and Fluid Flow - University of Florida
cao.chem.ufl.eduC. Fluid flow in open tubes: 1. Flow of a solvent through a circular tube can be one of three types (a) plug flow, (b) turbulent flow, or (c) laminar flow. 2. The type of flow expected in a given system can be determined by using the Reynolds number (Re): the ratio of inertial force and viscous force Re = ρ u dp/η Where: ρ = solvent density ...
Liquid Chromatography - University of Florida
cao.chem.ufl.eduLiquid Chromatography 1. Introduction and Column Packing Material 2. Retention Mechanisms in Liquid Chromatography 4. Column Preparation 6. Detectors (Chapter 4 and 5 in The essence of chromatography) 3. Method Development ... Resistance of mass transfer in stagnant mobile phase is a dominant term in mobile phase of LC. It is due to differences ...
Fluid Flow in Packed Beds - University of Florida
cao.chem.ufl.eduFluid Flow in Packed Beds: 1. Flow in a packed bed involves a complex pattern of solvent traveling in and around spaces in the support materials, through channels or pores of various sizes and shapes. 2. The basic structure of a packed bed is made up of three regions (a) Flowing mobile phase: the solvent which is flowing freely
Supercritical Fluid Chromatography - University of Florida
cao.chem.ufl.eduRefractive Index Detector (10-5to 10-6M) Absorption Detector (UV/Vis) (10-8M) Fluorescence Detector (10-10M) Conductivity Detector (10-6M) Electrochemical Detector (10-11M) GC detectors: d. Depending on which supercritical fluid is used, it is also possible to use SFC at lower T than GC. This makes it more useful in the
Related documents
Fundamentals of Probability
78bbm3rv7ks4b6i8j3cuklc1-wpengine.netdna-ssl.comthe theoretical probability. ... Now, to find the number of license plates that contain only one “A,” look at each space again. If the “A” were to appear in the first letter space, there is only one letter choice for ... The “n” is the total number of items, and the “r” is the number of ways they are being selected. Example: ...
Separation Ch. 23 Fundamentals of Analytical Separations ...
www.calstatela.eduNumber of Theoretical Plates • Example: A solute with a retention time of 407 s has a base width of 13 s on a 12.2 m column. Find the plate height and number of plates. 4 2 2 2 2 1.57 10 13 16 (16)(407) = = = × w t N r 0.78 mm 1.57 10 12.2 m 4 = × = = N L H Factors Affecting Resolution Increase resolution: – Increase column length (Square ...
A Guide to Validation in HPLC - PARAS'S PHARMACY WORLD
parasshah.weebly.comThe plate number depends on column length - ie the longer the column the larger the plate number. Therefore the column's efficiency can also be quoted as: Either- as the plate height (h), or the height equivalent to one theoretical plate (HETP). h= L where L = length of column n n Or- as plates/meter.
Gas Chromatography: Principles and Determination of ...
chemlab.truman.eduThe number of theoretical plates (n) of a column with respect to a particular compound can be found using the following equation: N = 16(tr/w) 2 = 5.55(t r/w1/2) 2 where w is the peak width measured in the same units as tr and w1/2 is the peak width measured at half of the peak height..
GC Column Selection Guide - Sigma-Aldrich
www.sigmaaldrich.com(number of theoretical plates) and sample capacity (amount of any one sample component that can be applied to the column without causing the desired sharp peak to overload). Optimizing one of these factors requires a sacrifice from the other. The ideal I.D. for a given application is dependent on the analytical needs.
Number of Theoretical Plates (N) - Agilent
www.agilent.comNumber of Theoretical Plates (N) Also known as column efficiency, the number of theoretical plates is a mathematical concept and can be calculated using Equation 4. A capillary column/any chromatography column does not contain anything resembling physical distillation plates or other similar features.
DESIGN OF WATER SUPPLY SYSTEM
designwss.weebly.comproportional to the surface area of the plates and to the velocity of the upper plate, and is inversely proportional to the distance between the plates. F: force in Newton [N] μ: dynamic viscosity [Pa·s] A: Surface of the plates [m2] v: velocity of the upper plate [m/s] y: distance between the plates [m] y A v F P Eq. 1-6 v y A F P Eq. 1-7