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Liquid Chromatography - University of Florida
Liquid Chromatography 1. Introduction and Column Packing Material 2. Retention Mechanisms in Liquid Chromatography 4. Column Preparation 6. Detectors (Chapter 4 and 5 in The essence of chromatography) 3. Method Development ... Resistance of mass transfer in stagnant mobile phase is a dominant term in mobile phase of LC. It is due to differences ...
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Supercritical Fluid Chromatography - University of Florida
cao.chem.ufl.eduRefractive Index Detector (10-5to 10-6M) Absorption Detector (UV/Vis) (10-8M) Fluorescence Detector (10-10M) Conductivity Detector (10-6M) Electrochemical Detector (10-11M) GC detectors: d. Depending on which supercritical fluid is used, it is also possible to use SFC at lower T than GC. This makes it more useful in the
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Diffusion and Fluid Flow - University of Florida
cao.chem.ufl.eduC. Fluid flow in open tubes: 1. Flow of a solvent through a circular tube can be one of three types (a) plug flow, (b) turbulent flow, or (c) laminar flow. 2. The type of flow expected in a given system can be determined by using the Reynolds number (Re): the ratio of inertial force and viscous force Re = ρ u dp/η Where: ρ = solvent density ...
Fluid Flow in Packed Beds - University of Florida
cao.chem.ufl.eduFluid Flow in Packed Beds: 1. Flow in a packed bed involves a complex pattern of solvent traveling in and around spaces in the support materials, through channels or pores of various sizes and shapes. 2. The basic structure of a packed bed is made up of three regions (a) Flowing mobile phase: the solvent which is flowing freely
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