coagulase-negative staphylococci

1 coagulase - negative staphylococci 1, 2) 1) 1) 1) 1) ! 1)1)" # $%&' ( *+2), % #- ' 0 ( * 216 4326 216 9327 coagulase - negative staphylococci (CNS) 937 8 ! < > CD E G G L MN BACTEC 9120 R CD Congo Red Agar ! 217( ) U X ( ) Z \] CD7 137 127 CD ^ ` Z b,2 d 157( ) E G G ef/ \] L CNS E G G \ i30 0 157 137 lm 2 3n o ! p q s o6 t7 u L Key words: coagulase - negative staphylococci , CD 8 coagulase - negative staphylococci (CNS) d:! ` ; w ; G G x< y 1)> #z> {| y E 2) ~ ~ B (percutaneous transluminal coronaryangioplasty; PTCA)| 3) \ CNS di \ CD 4) L CNS !}

2 R] I. 1. 8 $%&' ( n 210 L 1997 43 L2000 103 3 7 3 3,057 N L 518 N5617 O CNS 987 GQ* R \] 84ef937 8 \] 9ef 52 Q 42 3 T ] 7 U Staphylococcus epidermidis447 Staphylococcus hominis187 Staphylococcus capi-tis137 Staphylococcus haemolyticus67 Staphy-lococcus warneri57 Staphylococcus caprae47 Staphylococcus cohnii17 Staphylococcus hyicus17 Staphylococcus saprophyticus17 Q N-IDG SP-18 W R] 87 f< ( 901 2132) 4 16 1 % #- ' 0 ( * TEL: 098 878 0231 1149 FAX: 098 878 5593E-mail: D Vol. 14 No. 3 2. BACTEC 9120 1 resin #$ 7 & #' ( 70 * - Q #0 1 10 6 0 '78 : & < = ( 1 ?@ B 8 #' : IJ & ( & M N OP 8: 3.)))))

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4 14 No. 3 ) 2 " #$% & ( * , 3) ./ 1 23 ./ 1 23 45 78& 9: <= > = @ = @ = B=, & ( E 4)G I% J 3G I% J 3K , &Mann Whitney sUtest L( MN , &Fisher s exact probability test L( II. 1.#$% .3 .3 MQ 21M( ) 7M( ) 65M( ) S KU1 V X 2. " CRAY& 13MQ .3 K S. epidermidis X #$%&K \]^ b< c9d PTCAe c2d gh]^ b< c1d i %jke c1d 12dQ U2 .3 ( 3../ 1lm n K S. epidermidis44M 16M( ) .3 V U3 S. epidermidisK &o5 ( U2 " X qrsb& \]^ btkX , & &o5 ( 4. vw Kx2 V 30 y &{QX }~ 2 K , & & ( U2 "M(mean hour SD; )K U2#$% ./ 1lm MN MN(R) S. epidermidis " X qrsb& \]^ btkX 2116 ( ) 112 ( )12 ( ) 215 ( ) 4 73( )1 ( )4 ( )4 ( ) 5 6525 ( ) 10( )13 ( ) 214 ( ) 4, 5 1 5: N Kq V G I% J 1,P ; 2, ; 3,P ; 4,P ;5,P Mean hour SDx2 & U3 n MN MN MN(R)S.)))))

5 Epidermidis4416 ( )S. hominis181 ( )S. capitis132 ( )S. hoemolyticus61( )S. warneri51( )S. caprae40( )S. cohnii10( )S. hyicus10( )S. saprophyticus10( ) CNS L( ./ 1 23 CNS L( ./ 1 23 #$ " I Vol. 14 No. 3 ( ) (P ) 5. " 4 ) *+,- 10 *+/ 07) * * 4 5 ) 89: 10 24 30 36 III. A BC 8: F4 !GHI CNS5 M " Staphylococcus aureus" O P Q CNS5 % ST U * W XY W Z"[ ] ^ 4 _ 5`+" a ,b _ c-^ d j9 "k * l * /n * BC * CNS 1 38) 3 OoB 56 5) ica mecA 9) s * 3O56 * 1 5 1 * Q x z 10, 11) 8 BZ"{ ^|* } ~ 5BC : < > ?

6 3 OoB B * M XY 5 3 OoB `+ 4 1" 5 * z EF BC * CNS I J * } 5 BC * CNS 5K * L* * 5 K *U L* * MN * O MN" O^ BC * CNS "R " CNS Tx 89: 4) * 89: 5 89: B X ! [ ] _" ^ 5 BaW Z "[ ] ^12) 89: "/ ^ 1 PCR1 89: ica I P 13) * O CRA15 Of gQ^ 13) " d ef h e 1 Christensen 114) I P 6) i1"oj O ,b * 18 k 5^ 5 BaW _ z 10 ( ) 89: Ar-ciola 13)( ) l 89: 13 12 * 2 CRA15 CNS 4 M W Nn N N N 24 89: 24 30 36 i,bp Vol.

7 14 No. 3 2004. M s v w M s v w 52180 2 30 2 $ % 2 ($) 15) -.$ ( /0 12 $ % 4 % $7 $7 8 9 12 $ % < 12 0 = @ B 7 B 7 C % @ B 7 DE" # J@KLM N 1) O & Q D * 8 SD , $, - 0 @ B 7 % SD $, @ B V16) S. epidermidis X $) Q YZ @ B 7 [ S. epidermidis 0 0 S. epidermidis 2Q $ \] 0 2 $) _ ` S. epider-midis 2Q @ B 7 \] 12 $ 5 $ 7bc de 4 @ B 7 100h : 0 iXj7 k l m= @ B dn pq$ r sQ @ B = t u2 Q vw x ? z j7t @} ~ A : 10 bc : t k D EF m30 " j7 l bc ]G 157 H 137 $ 17 $ 17 I$ bc ]G 0 H $ 87 YZ D EF L $ 7bc D X D ) # = PTCA ND I _ k H 7 0 $ D EF m30 " j7 $ 7bc Q D SD UD VF} W 0 - Q17) H L 5 L s 5 - \ 24 365 / L 5 7 ] D CNS $ 7bc = Y $ 7bc Q L 5 r IV.

8 D CNS $ 7bc D EF 30 " $7 bc 937 157 u 137 pq$ H j7 bc 1a 1) Huebner, J., D. A. Goldmann. 1999. coagulase - negative staphylococci : role as Rev. Med. 50: 223 )b c 1987. d I @(b) $ I @w13: 471 ) Samore, M. H., M. , S. M. Lewis,et al. 1997. Frequency, risk factors, and out-come for bacteremia after percutaneous trans-luminal coronary angioplasty. Am. J. : 873 ) Peters, G. 1988. New considerations in thepathogenesis of coagulase - negative staphylo-coccal foreign body infections. J. 21: 139 ) Souvenir, D., D. E. Anderson, S. Palpant, et Blood cultures positive for coagulase - negative staphylococci : antisepsis, pseudobac-teremia, and therapy of patients. J. Clin. Micro-biol. 36: 1923 ) Freeman, D. J., F. R. Falkiner, C. T. New method for detecting slime produc-tion by coagulase negative staphylococci .

9 Pathol. 42: 872 CNS $ 7bc D CNS $ 7bc H w QVol. 14 No. 3 ) 2003. 4: 153 ) Kirchho#, , J. N. Sheagren. 1985. Epidemi-ology and clinical significance of blood cul-tures positive for coagulase - negative staphylo-coccus. Infect. Control 6: 479 ) Frebourg, N. B., S. Lefebvre, S. Baert, et PCR-based assay for discrimination be-tween invasive and contaminatingStaphylococ-cus epidermidisstrains. J. Clin. Microbiol. 38:877 ) Mirrett, S., M. P. Weinstein, L. G. Reimer, et Relevance of the number of positive bot-tles in determining clinical significance of co-agulase- negative staphylococci in blood cul-tures. J. Clin. Microbiol. 39: 3279 ) 2004. !"# '() 53: 1127 )+ , / 0 1 epidermidis slime3456 bio-film9:; ?(6@ B CD 65: 875 ) Arciola, C. R., L. Baldassarri, L.

10 Presence oficaAandicaDgenes andslime production in a collection of staphylococ-cal strains from catheter-associated Clin. Microbiol. 39: 2151 ) Christensen, G. D., W. A. Simpson, A. L. Bisno,et al. 1982. Adherence of slime-producingstrains ofStaphylococcus epidermidisto smoothsurfaces. Infect. Immun. 37: 318 ) FGH J MN 2004. ! T WX [ )^_ 6bc d 53: 22 ) Levy, M. F., D. D. Schmitt, D. D. Edmiston, et Sequential analysis of Staphylococcalcolonization of body surfaces of patients un-dergoing vascular surgery. J. Clin. : 664 )ef h i k m r !st vw yw z |4} 6 c 6@ B |4} 12: 86 of criteria for determining clinically significant bacteria usingthe time to positive culture of coagulase - negative staphylococciin blood culturesTakeya Ohshiro,1, 2)Kaoru Uchima,1)Ryouko Ohshiro,1)Orie Ohshiro,1)Hideji Taira,1)Kouhachi Oyakawa1)1)Department of Clinical Laboratory, Northern part of Okinawa Medical Association Hospital2)Present address for corresponding: Department of Clinical Laboratory, Urasoe General HospitalNinety-three strains of coagulase - negative staphylococci (CNS) were used to determine the criteria fordetermining clinically significant bacteria based on the time to positive culture, positive slime production,and the presence of an indwelling central venous catheter.]