TRC TRCRapid M. TB - jscm.org

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7 18 No. 1 / TRC / 1717 770 1,000 ! Q$ & '( ) *1,000 +, - /0 3*TRC4 6 7 :;(* ?@ABC PCRF H J 7 M*N J TRCF RST U W J T7 4 YZH J [ T\T J Z `a*Nb cd 3 7 4 J T * 3 Re f g T f J ! Q$ & h 4 ij J [ Yl T J *mn +, pqF rs( ( v wx Yyz m {|T\ } a ~ \ ! Q$ & '( ) j J * - T 4 7 3 d? * U '( * T\ U T U Q$ qF PCRF v ( U \J * 1) Tenover, F. C., J. T. Crawford, R. E. Huebner, etal. 1993. The resurgence of tuberculosis: isyour laboratory ready?))))]]

8 J. Clin. Microbiol. 31:767 ) Jensen, P. A., L. A. Lambert, M. F. Guidelines for preventing the transmis-sion ofMycobacterium tuberculosisin health-care settings, 2005. MMWR Recomm. Rep. 54:1 ) Ishiguro, T., J. Saitoh, R. Horie, et al. activating fluorescence DNAprobe and its application to homogeneousquantification of a target sequence by isother-mal sequence amplification in a closed Biochem. 314: 77 ) Ishiguro, T., J. Saitoh, H. Yawata, et al. detection of specific sequence ofnucleic acids by oxazole yellow-linked oligonu-cleotides. Homogeneous quantitative monitor-ing ofin vitrotranscription. Nucleic Acids : 4992 ) Takakura, S.

9 , S. Tsuchiya, Y. Isawa, et al. detection ofMycobacteriumu tuberculosisin respiratory samples by transcription-reversetranscription concerted reaction with an auto-mated system. L. Clin. Microbiol. 43: 5435 ) Nakaguchi, Y., T. Ishizuka, T. Hayashi, et Rapid and specific detection of tdh, trh1,and trh2 mRNA ofVibrio parahaemolyticusbytranscription-reverse transcription concertedreaction with an automated system. Clin. Mi-crobiol. 42: 4284 ) Ishii, T., Y. Fujiwara, S. Ohnaka, et al. genetic diagnosis with the transcrip-tion reverse transcription concerted reactionsystem for cancer micrometastasis. Ann. 11: 778 ) Kaito, K., H. Otsubo, S.

10 Takahara, et al. antigen (CEA)-producingmultiple myeloma detected by transcription reverse transcription concerted reaction sys-tem. Int. J. Hematol. 85: 128 1319) Y 2002 e 46: 3 ) Takakura, S., S. Tsuchiya, N. Fujihara, et Isothermal RNA sequence amplificationmethod for rapid antituberculosis drug suscep-tibility testing ofMycobacterium Microbiol. 43: 2489 2491. p } Vol. 18 No. 1 2008. 1818 Transcription Reverse Transcription Concerted Reaction in DetectingMycobacterium tuberculosis(TRCR apid M. TB)Taku Tamura, Kenji Tominaga, Kazumi Sakamoto, Harumi Tsurukawa,Saori Maeda, Mariko Wakabayashi, Mitsutaka Sasaki,Tomoko Ishikawa, Ikurou Abe, Ken KaitoCentral Clinical Laboratory, Jikei University Hospital,3 19 18 Nishishinbashi, Minato-ku, Tokyo 105 847, JapanTranscription reverse transcription concerted reaction system (TRC system) is a new method for theamplification and the detection of the target RNA, and it is recently introduced to the rapid detectionsystem forMycobacterium tuberculosisas TRCR apid M.