Transcription of Competent Quick DH5α - TOYOBO
1 DNA-913 - 0 - 21-04 - Competent Cell - Competent Quick DH5 (Code No. DNA-913) 1 1 A3981K DNA-913 - 1 - [ ] 2 10 [ ] Competent Quick DH5 100 L 20 [ ] 1pg pBR322 LB/ 1 108 transformants / g pBR322 - pUC19 LB/ /X-gal 99% [ ] -80 C -80 C 3 3 1 [ ] Genotype DH5 deoR, endA1, gyrA96, hsdR17(rk-,mk+), phoA, recA1, relA1, supE44, thi-1, (lacZYA-argF)
2 U169, 80dlacZ M15, F-, - - 2 - DNA-913 [ ] SOC 1/3 SOC 1/3 1/10 1 SOC SOC Competent high DH5 Code Competent Quick DH5 1 DNA 2 5 42 C 30 LB + 3 37 C 1 37 C DNA 2 DNA 30% DNA 100 L 10ng 3) LB 50 100 g/ mL 10 50 g/ mL X-gal 40mg/mL ( ) X-gal 20 L LB 4 50% - 3 - 1 pBR322(Ampicillinr, Tetracycliner) pAK119M(Kanamycinr) pAT5 Chloramphenicolr 10pg / 5 L TE buffer SOC 37 C 30 60 10 108 transformants / g plasmid SOC 108 LB (Amp) 50 g/mL (Kan) 10 g/mL (Tet) 20 g/mL (Cm) 100 g/mL 2 pBR322(Ampicillinr)
3 5pg / 5 50 L TE buffer DNA 30% + + + +090102030405060 100 plasmid ( ) transformants/ g pBR322) + + + +09 +SOC 30min +SOC 60min transformants/ g plasmid AmpKanTetCm - 4 - DNA-913 [ ] Hanahan D., J. Mol. Biol., 166, 557 (1983) Inoue H., Nojima H., and Okayama H., Gene, 96, 23-28 (1990) [ ] * Code No. Competent high JM109 < E. coli JM109 SOC > 100 L 10 DNA-900 Competent high DH5 < E. coli DH5 SOC > 100 L 10 DNA-901 Competent high DH5 < E. coli DH5 SOC > 100 L 10 DNA-903 *SOC 1mL 10 pBR322 3 pBR322(Ampicillinr) 1pg 100ng / 5 L TE buffer DNA DNA 100 L 10ng + + + + 100 l plasmid transformants/ g pBR322 0 1 10 100 1 10 100pg pg pg ng ng ng 1 ( ) 530-8230 2 8 TEL 06-6348-3786 FAX 06-6348-3833 E-mail : ( ) 104-8345 17 10 TEL 03-6887-8819 FAX 03-6887-8951 E-mail : TEL 06-6348-3888 FAX 06-6348-3833 9:00 12:00 , 13:00 17:00 ( ) E-mail : URL
