KOD －Plus－ - TOYOBO

1 (KOD-201) TEL 06-6348-3888 FAX 06-6348-3833 9:00~12:00, 13:00~17:00 ( ) E-mail: [URL] Code No. KOD-201 Code No. KOD-201X5 Code No. KOD-201X10 C KOD - plus - Thermococcus kodakarensis KOD1 KOD DNA Polymerase1,2) PCR KOD DNA Polymerase 2 Hot start PCR KOD DNA Polymerase Polymerase 3 5 Exonuclease (Proofreading ) PCR KOD DNA Polymerase Taq DNA Polymerase 82 KOD DNA Polymerase 2 KOD Polymerase Exonuclease PCR PCR 1.

2 KOD- plus - 3 KOD-201 (200U 1 ) KOD-201X5 KOD-201X10 KOD - plus - ( U/ L) 200 L 1 (KOD-201) 5 (KOD-201) 10 10 Buffer for KOD - plus - 1mL 1 25mM MgSO4 1mL 1 2mM dNTPs 1mL 1 2. 3. 1 KOD - plus - PCR 50 L 1 L(1U) KOD - plus - 1U DNA 21kb Genomic DNA 12kb 7kb 2 KOD - plus - Human -globin Human p53 22-05 (KOD-201) TEL 06-6348-3888 FAX 06-6348-3833 9:00~12:00, 13:00~17:00 ( ) E-mail: [URL] 4.

3 PCR 1 PCR 10 PCR Buffer for KOD - plus - Buffer Components Volume Final Concentration 10 PCR Buffer for KOD - plus - 5 L 1 2mM dNTPs 5 L each 25mM MgSO4 2 L (10 M each) L M each DNA 1 L Genomic DNA 10~200 ng/50 L Plasmid DNA 1~50 ng/50 L cDNA KOD - plus - (1U/ L) 1 L 1 U/50 L Autoclaved,distilled water to 50 L 2 PCR 3 Tm (72 C ) PCR 2 3 2 Pre Denature : 94 C, 2min. Pre Denature : 94 C,2min. Denature : 94 C, 15sec.

4 Denature : 94 C,15sec. Annealing : (Tm-5) C, 30sec. Extension : 68 C,1min. /kb Extension : 68 C, 1min. /kb (Extension) 1kb 1min. 5. 1 thin-wall 2 3 dNTPs dNTPs Mixture(2mM) (Code ) 4 GC 22~34mer (Tm >60 C) 5 PCR Tm 65 C 6 DNA PCR DNA DNA RNA PCR 25 35 cycles 25 35 cycles (KOD-201) TEL 06-6348-3888 FAX 06-6348-3833 9:00~12:00, 13:00~17.

5 00 ( ) E-mail: [URL] 7 DNA RNA PCR PCR 50 L RNA 100ng Total RNA1 g 20 L PCR 2 L NA RNA RNA DNA DNA RNase 8 GC rich DMSO 2~5% 9 PCR DNA DNA 50~200ng 30~40 10 Colony Direct PCR PCR PCR 11 PCR blunt end( ) PCR blunt end KOD/KOD- plus - TA TArget CloneTM - plus -( ) TA Nearest Neighbor method Tm ( ) 6.

6 MgSO4 ~ Tm-10 C +2~5 DNA, DNA RNA RNA RNA 68 C PCR MgSO4 2~5 DNA MgSO4 1U ~ TA TArget CloneTM - plus - 10 Buffer for KOD - plus - (KOD-201) TEL 06-6348-3888 FAX 06-6348-3833 9:00~12:00, 13:00~17:00 ( ) E-mail: [URL] 7.

7 1 DNA Polymerase p53 4kb PCR PCR DNA Polymerase DNA 50 100ng KOD - plus - M 1 2 3 4 5 6 M 2 TGF- (GC: 70%) DMSO DNA TGF- (GC: 70%) 2kb 30 PCR DMSO M 1 2 3 ( ) DMSO KOD DNA Polymerase M: /Hindlll Marker 1: KOD - plus -, DNA 50ng 2: KOD - plus -, DNA 100ng 3: A , DNA 50ng 4: A , DNA 100ng 5: B , DNA 50ng 6: B , DNA 100ng M: 1kb 1: KOD - plus - 2: KOD - plus - + 2%DMSO 3: KOD - plus - + 5%DMSO (KOD-201) TEL 06-6348-3888 FAX 06-6348-3833 9:00~12:00, 13:00~17:00 ( ) E-mail: [URL] 8.

8 <M-MLV > ReverTra Ace (100 U/ L) 10,000 U 1 (10,000 U 1 ) 5 (10,000 U 1 ) 10 TRT-101 TRT-101X5 TRT-101X10 < PCR > KOD DNA Polymerase 250 U 1 (250 U 1 ) 5 KOD-101 KOD-101X5 < PCR > KOD 200 U 1 (200 U 1 ) 5 KOD-211 KOD-211X5 < PCR > KOD - plus -Neo 200 U 1 (200 U 1 ) 5 (200 U 1 ) 10 KOD-401 KOD-401X5 KOD-401X10 < KOD/KOD - plus - TA > TArget CloneTM - plus - 10 TAK-201 dNTPs Mixture(2mM) 1 mL NTP-201 KOD - plus - Buffer 1 mL KOD-2B KOD - plus - MgSO4 1 mL KOD-2S KOD - plus - Buffer 1 mL KOD-3B KOD - plus - Neo Buffer 1 mL KOD-4B 9. (1)Takagi, M., Nishioka, M., Kakihara, H., Kitabayashi, M., Inoue, H., Kawakami, B., Oka, M., and Imanaka, T., Appl. Environ. Microbiol.

9 , 63, 4504-4510 (1997) (2)Hashimoto, H., Matsumoto, T., Nishioka, M., Yuasa, T., Takeuchi, S., Inoue, T., Fujiwara, S., Takagi, M., Imanaka, T., and Kai, Y., J. Biochem. (Tokyo), 125, 983-986 (1999) (3)Mizuguchi, H., Nakatsuji, M., Fujiwara, S., Takagi, M., and Imanaka, T., J. Biochem. (Tokyo), 126, 762-768 (1999) (KOD-201) ( ) 530-0001 13 1 TEL 06-6348-3786 FAX 06-6348-3833 E-mail : ( ) 104-8345 17 10 TEL 03-6887-8819 FAX 03-6887-8951 E-mail.