E-Gel® Technical Guide - Thermo Fisher Scientific

Thermo Scienti˜ c TSQ Quantiva Triple-Stage Quadrupole ...

Thermo Scienti˜ c TSQ Quantiva Triple-Stage Quadrupole Mass Spectrometer ... • Thermo Scientifi c ... New Zealand +64 9 980 6700 Norway +46 8 556 468 00 Russia/CIS +43 1 333 50 34 0 Singapore +65 6289 1190 Spain +34 914 845 965 Sweden +46 8 556 468 00 Switzerland +41 61 716 77 00

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Acetone precipitation of proteins - Thermo Fisher Scientific

• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins might denature, making the pellet difficult to re-solubilize.

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Ni-NTA Purification System - Thermo Fisher Scientific

7 Introduction Overview Introduction The Ni-NTA Purification System is designed for purification of 6xHis-tagged recombinant proteins expressed in bacteria, insect, and mammalian cells. The system is designed around the high affinity and selectivity of Ni-NTA Agarose

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TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A

TRIzol™ Reagent Catalog Numbers 15596026 and 15596018 Doc. Part No. 15596026.PPS Pub. No. MAN0001271 Rev. A.0 WARNING! Read the Safety Data Sheets (SDSs) and follow the

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Interpaion IEnPp fR nmpmeft n CfhRn Labware Chemical ...

286 www.thermoscientifi c.com Labwre Olafira nOPao ymreblo LsFoOrao LuapeDisEHLFbDabwriweFb CMT TTLy TL TM TT T/C T/ / ewFb TT na as H MnCM TM TTM Chemhical,l contaroAcSnlno#E N,o u u b u b b M M M b u u .

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Interpretation of Nucleic Acid 260/280 Ratios

T123 – TECHNICAL BULLETIN NanoDrop Lite Interpretation of Nucleic Acid 260/280 Ratios T123– Rev 1/2012 Thermo Scientific NanoDrop Products Wilmington, Delaware USA Technical support:nanodrop.techsupport@thermofisher.com

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Corona CAD Charged Aerosol Detector - Thermo Fisher …

3 Corona CaD DeteCtor SpeCifiCationS* Operating Mode Charged Aerosol Detection Mobile Phase Flow Rate Up to 2 mL/min Full Scale Output Range 1 pA to 470 pA

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GeneAmp PCR System 9700 - Thermo Fisher Scientific

Introduction and Safety 1-1 Introduction and Safety 1 Overview About This Chapter This chapter provides information to help you safely operate the GeneAmp PCR System 9700. In This Chapter The following topics are covered in this chapter: Topics See Page About This Manual 1-2

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alamarBlue TI read only - Thermo Fisher Scientific

Page 1 of 27 alamarBlue® Assay U.S. Patent No. 5,501,959 Indications for Use The alamarBlue® Assay is designed to measure quantitatively the proliferation of various human and animal cell lines, bacteria and fungi. The bioassay may also be used to establish relative

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Installation Guide -Chromeleon 7 - Thermo Fisher Scientific

Note: Indicates information of special interest. Tip: Indicates information that will help you to use the software more efficiently. 1.3 Other Documents

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Lab 4: Gel Electrophoresis - Vanderbilt University

Gel electrophoresis is a method of separating DNA fragments by movement through a Jello-like substance called agarose. Derived from a seaweed polysaccharide, agarose gels form small pores that act as sieves to separate DNA based on …

The GelBottle Inc (TGB) Gel Polish / Builder in A Bottle ...

GEL POLISH COLOUR STEP #3: FIRST LAYER - GEL POLISH COLOUR STEP #4: SECOND LAYER - GEL POLISH COLOUR STEP #5: TOP COAT - GEL POLISH COLOUR • Apply the first layer of a TGB Gel Polish Colour of your choice and seal the free edge. • Cure using the TGB Light The Way Lamp for 60 seconds, the lamp has been specifically designed to cure TGB …

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Gel Filtration - Harvard University

Gel filtration is a robust technique that is well suited to handling biomolecules that are sensitive to changes in pH, concentration of metal ions or co-factors and harsh environmental conditions. Separations can be performed in the presence of essential ions or cofactors,

QIAquick Gel Extraction Kit Protocol using a microcentrifuge

QIAquick Gel Extraction Kit Protocol using a microcentrifuge This protocol is designed to extract and purify DNA of 70 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. Up to 400 mg agarose can be processed per spin column. This kit can also be used for DNA cleanup from enzymatic reactions (see page 8).

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Gel Electrophoresis: How Does It Work - Purdue University

gel The gel is held in the casting tray. It provides a place to put the small particles you wish to test. The gel contains pores that allow the particles to move very slowly toward the oppositely charged side of the chamber. At first, the gel is poured in the tray as a hot liquid. As it cools, however, the gel solidifies. comb

Recommended SDS PAGE Stain Protocols - UF Chemistry

2. Wash the gel with 3 aliquots of water, shaking for 5 mins each. 3. Stain the gel in Gel-Code Blue stain Reagent for 1 hour, gently rock at room temperature. 4. Wash the gel with ddH2O, shake about 2-3 hours, change water 3 to 4 times. 5. Store gel in 5% acetic acid solution at 4°C until in-gel digestion is performed (Gel can be

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