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GE Healthcare - Fred Hutch
Charge Ion exchange chromatography (IEX) Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Biorecognition (ligand specificity) Affinity chromatography (AC) Fig I.1. Separation principles in chromatographic purification.
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Quantitative Real Time PCR Protocol - Fred Hutch
research.fredhutch.org1. For each cDNA reaction, make a 1:100 dilution of cDNA into RNase-free dH 2O. NOTE: Working cDNA dilution depends on abundance of transcript so it may be necessary to make a dilution series (e.g. 1:10, 1:100, 1:1000, 1:10,000) to determine optimum cDNA input dilution. 2.
pET System Manual - Fred Hutch
research.fredhutch.orgLigation-independent cloning was developed for the directional cloning of PCR products without restriction enzyme digestion or ligation reactions (Aslanidis and de Jong, 1990; Haun et al., 1992). LIC vectors are created by treating a linearized backbone with T4 DNA polymerase in the presence of only one dNTP. The 3'→5' exonuclease activity of ...
SPRI Based Size Selection - Fred Hutch
research.fredhutch.orgratio or weaker magnets will require a longer settle time. Remove and discard the clear supernatant. NOTE Care should be taken not to aspirate more than a trace amount of beads during this step, as the desired library is associated with the beads. Significant bead loss will result in reduced yield. 4.
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Size Exclusion Chromatography
wolfson.huji.ac.ilSize Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Charge Ion exchange chromatography (IEX) Biorecognition (ligand specificity) Affinity chromatography (AC) Isoelectric point (pI) Chromatofocusing (CF) Fig I.1.
An Introduction to Gas Chromatography Mass Spectrometry
www.ucl.ac.ukThe two principal types of chromatography are gas chromatography (GC) and liquid chromatography (LC). Gas chromatography separates gaseous substances based on partitioning in a stationary phase from a gas phase. Liquid chromatography includes techniques such as size exclusion (separation based on molecular size), ion
Separation techniques: Chromatography
jag.journalagent.commechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatogra-phy is one of the most common methods of protein purification.
Shimadzuʼs Fundamental Guide to Gas Chromatography …
www.shimadzu.eu.comSEC Size Exclusion Chromatography SIM Selected Ion Monitoring SRM Selected Reaction Monitoring TIC Total Ion Current TOF Time-of-Flight TQ Triple Quadrupole UFMS Ultra-Fast Mass Spectrometry UHPLC Ultra-High-Performance Liquid Chromatography UV-VIS Ultraviolet-Visible Spectroscopy 6. Symbols. µ Micro amu Atomic mass unit ...
Basic Guide to Chromatography - University of San Diego
home.sandiego.edusize exclusion resins, then you must have a taller thin column. The specific amount of resin depends on the type of chromatography and the particular binding capacity of each resin. For resins that bind their analyate, the top 20% of the column should bind most of the protein, for SEC columns, the sample volume loaded should be no more
Waters 2414 Refractive Index Detector
www.waters.comanalyses by gel-permeation or size-exclusion chromatography. RI detectors work by measuring changes in the bulk properties of the solvent and the solutes under investigation, exploiting the improbability that solvents . Refractive index Refractive index) = …