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Shimadzuʼs Fundamental Guide to Gas Chromatography …
SEC Size Exclusion Chromatography SIM Selected Ion Monitoring SRM Selected Reaction Monitoring TIC Total Ion Current TOF Time-of-Flight TQ Triple Quadrupole UFMS Ultra-Fast Mass Spectrometry UHPLC Ultra-High-Performance Liquid Chromatography UV-VIS Ultraviolet-Visible Spectroscopy 6. Symbols. µ Micro amu Atomic mass unit ...
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Basics & Fundamentals - Gas Chromatography
www.shimadzu.eu.comGas chromatography (GC) is an analytical methodology, which was devised by Nobel Laureate, Martin, et al. in 1952. More than 60 years after the award, GC systems are widely commercialized and used in various industries, capable of both of quantitation and qualifcation. GC is applicable for many types of analysis in the markets such as residual
Tips for practical HPLC analysis
www.shimadzu.eu.comHPLC-type acetonitrile has the lowest absorbance (particularly for short wavelengths). Using an organic solvent with lower absorbance as the mobile phase results in less noise in UV detection, and so HPLC-type acetonitrile is suitable for high-sensitivity analysis in the UV short-wavelength range. Also, using HPLC-type acetonitrile
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Size Exclusion Chromatography
wolfson.huji.ac.ilSize Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Charge Ion exchange chromatography (IEX) Biorecognition (ligand specificity) Affinity chromatography (AC) Isoelectric point (pI) Chromatofocusing (CF) Fig I.1.
An Introduction to Gas Chromatography Mass Spectrometry
www.ucl.ac.ukThe two principal types of chromatography are gas chromatography (GC) and liquid chromatography (LC). Gas chromatography separates gaseous substances based on partitioning in a stationary phase from a gas phase. Liquid chromatography includes techniques such as size exclusion (separation based on molecular size), ion
GE Healthcare - Fred Hutch
research.fredhutch.orgCharge Ion exchange chromatography (IEX) Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC) Biorecognition (ligand specificity) Affinity chromatography (AC) Fig I.1. Separation principles in chromatographic purification.
Separation techniques: Chromatography
jag.journalagent.commechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatogra-phy is one of the most common methods of protein purification.
Basic Guide to Chromatography - University of San Diego
home.sandiego.edusize exclusion resins, then you must have a taller thin column. The specific amount of resin depends on the type of chromatography and the particular binding capacity of each resin. For resins that bind their analyate, the top 20% of the column should bind most of the protein, for SEC columns, the sample volume loaded should be no more
Waters 2414 Refractive Index Detector
www.waters.comanalyses by gel-permeation or size-exclusion chromatography. RI detectors work by measuring changes in the bulk properties of the solvent and the solutes under investigation, exploiting the improbability that solvents . Refractive index Refractive index) = …