Example: bankruptcy

HPLC Column Selection

Analytical hplc HPLC Column Selection A Comparison of Reversed-Phase columns Based on the widely accepted work and data of Drs. Lloyd Snyder and John ,2 Grace has developed this Column Selection tool for choosing reversed phase HPLC columns based on peak capacities and Column selectivity of polar and nonpolar compounds. Typically, chromatographers choose HPLC columns by comparing physical characteristics, such as surface area and carbon load. Often, this does not provide enough information about selectivity or capacity for adequate Column Selection . This chart provides a hplc columns | introduction reliable means of choosing HPLC columns based on acidic, basic, and hydrophobic character.

www.discoverysciences.com 27 analytical hplc hplc columns | introduction Grace (Jones) Genesis® 120 AQ C18 A 0.960 Macherey Nagel Nucleodur® Pyramid 0.958 Thermo/Hypersil Hypersil® Elite C18 ®0.958

Tags:

  Selection, Columns, Column selection, Hypersil, Hypersil hypersil

Information

Domain:

Source:

Link to this page:

Please notify us if you found a problem with this document:

Other abuse

Transcription of HPLC Column Selection

1 Analytical hplc HPLC Column Selection A Comparison of Reversed-Phase columns Based on the widely accepted work and data of Drs. Lloyd Snyder and John ,2 Grace has developed this Column Selection tool for choosing reversed phase HPLC columns based on peak capacities and Column selectivity of polar and nonpolar compounds. Typically, chromatographers choose HPLC columns by comparing physical characteristics, such as surface area and carbon load. Often, this does not provide enough information about selectivity or capacity for adequate Column Selection . This chart provides a hplc columns | introduction reliable means of choosing HPLC columns based on acidic, basic, and hydrophobic character.

2 The Snyder/Dolan Column test procedure has been described in a series of publications. Based on retention data for a series of standard mixtures and the same separation conditions (50% acetonitrile/buffer; pH and ; 35 C), reversed-phase columns are characterized by five Column -selectivity parameters: hydrophobicity (H), steric interaction (S*), hydrogen-bond acidity A , basicity (B), and relative silanol ionization or cation-exchange capacity C . Here we have chosen to graphically highlight data for H , A , and C , with C results at pH Hydrophobicity (H) is often the primary analyte interaction with reversed phase columns and indicates overall capacity.

3 Secondary interactions are often polar interactions with basic analytes. The degree of unprotonated base interaction A and protonated base interaction C with the packing material is measured and represented here. Directions for Using the Column Chart Key to Chart The chart lists the columns in descending order of hydrophobic capacity (H) . To find similar HPLC columns to test as Hydrophobic Indicator back-up columns , follow these steps. A Hydrogen bonding Indicator pH 1) Find the Column you are currently using and note neighboring columns which have similar (H) capacity factors.

4 (Protonated under 2) Compare the values for interaction of polar compounds ( A and C ). acidic conditions). If there is more than one choice for a back-up Column , then compare your actual sample to the test probes. If your C Cation Exchange sample is nonpolar, then place more emphasis on hydrophobic values. If your sample is basic (polar), then pay special Indicator pH attention to A and C and determine if your sample will be protonated ( A ) or unprotonated ( C ) and place greater (Unprotonated under emphasis on one of these values. neutral conditions). Manufacturer Column Selectivity Parameters Manufacturer Column Selectivity Parameters ZirChrom ZirChrom -PBD C18 A C Merck LiChrospher 100 RP-18 C A YMC J'Sphere H80 C18 C A Bischoff ProntoSIL 120-5-C18 H C A Restek Allure C18 C A Shiseido CAPCELL C18 M G C A Phenomenex Ultracarb ODS (30) C A Grace (Jones) Genesis 120 C18 C A YMC YMC Pack Pro C18 RS C A Bischoff EU Reference Column C18 C A Grace (Alltech) Adsorbosphere UHS C18 C A Grace (Alltech)

5 Allsphere ODS2 CA Thermo/ hypersil hypersil BetamaxNeutral C18 C A Merck Purospher STAR RP18e C A Agilent Zorbax Extend C18 C A Merck Chromolith RP18e C A Agilent Zorbax C18 C A Phenomenex Luna C18(2) C A Beckman Ultrasphere ODS C A Varian Pursuit C18 C A Grace (Alltech) Alltima HP C18 High Load C A MacMod/ACT ACE 5 C18 C A Agilent Zorbax Rx-18 C A Tosoh TSKgel Super-ODS C A Agilent Zorbax Eclipse XDB-C18 C A Agilent Zorbax StableBond 80A C18 C A Supelco Ascentis C18 C A Phenomenex Prodigy ODS(2) C A Macherey Nagel Nucleodur C18 Gravity C A Thermo/ hypersil hypersil BDS C18 C A Grace (Grom) Grom Sapphire 110 C18 C A Grace (Alltech) Alltima C18 C A Restek Restek Ultra C18 C A Grace (Vydac) Vydac Everest C18 C A Varian OmniSpher 5 C18 C A Thermo/ hypersil hypersil Beta Basic-18 C A Grace (Vydac) Denali 120 C18 C A GL Sciences Inertsil ODS-3 C A Waters Symmetry C18 C A Grace (Alltech) Adsorbosphere C18 A C Akzo Nobel Kromasil 100-5C18 C A Phenomenex Synergi Max-RP C18 C A Waters Nova-Pak C18.

6 C A Shiseido CAPCELL C18 SG120 C A Thermo/ hypersil hypersil 100 C18 C A Grace (Jones) Apex I C18 A C MacMod/ACT ACE 5 C18-HL C A Thermo/ hypersil hypersil ODS-2 C A ZirChrom ZirChrom -EZ C18 A C Grace (Alltech) Alltima HP C18 C A Grace (Grom) Grom Sil 120 ODS-5 ST C A Waters Xterra MS C18 C A Dionex Acclaim 120 C18 C A Waters Symmetry 300 C18 C A Waters Sunfire C18 C A Supelco Discovery C18 C A Agilent Zorbax Eclipse Plus C18 C A Supelco Supelcosil LC-18-DB C A Merck Superspher 100 RP-18e . C A Waters Spherisorb S5 ODSB.. AC Shiseido CAPCELL C18 AG120 C A Thermo/ hypersil hypersil Bio Basic-18 C A Grace (Grom) Grom Sil 120 ODS-3 CP C A Thermo/ hypersil hypersil ODS AC Waters Delta-Pak C18 100A C A Bischoff ProntoSIL 120-5-C18-AQ C A Macherey Nagel Nucleodur Isis C A Grace (Jones) Genesis 300 C18 C18 C A Phenomenex Prodigy ODS (3)

7 C A Bischoff Prontosil 200-5-C18 AQ C A Phenomenex Synergi Hydro-RP C18 C A Agilent Zorbax C8 C A Phenomenex Luna C18 C A Tosoh TSK gel ODS-80Ts A C Supelco Supelcosil LC-18 A C Waters Resolve C18 A C YMC YMC Pro C18 C A Phenomenex Gemini C18 110A C A Phenomenex Onyx Monolithic C18 C A Grace (Alltech) Econosil C18 C A Bischoff ProntoSIL SpheriBOND 80-5-ODS2 C A Phenomenex Aqua C18 C A Grace (Jones) Apex II C18 A C YMC YMC ODS-AQ C18 C A Shiseido CAPCELL C18 UG120 C A Waters Spherisorb ODS-2 AC GL Sciences Inertsil ODS-2 C A Macherey Nagel Nucleosil 100-5-C18 HD C18 C A 26 analytical hplc Grace (Jones)

8 Genesis 120 AQ C18 C A Macherey Nagel Nucleodur Sphinx RP AC Macherey Nagel Nucleodur Pyramid C A Thermo/ hypersil Aquasil C18 C A Thermo/ hypersil hypersil Elite C18 C A MacMod/ACT ACE AQ EP C A Dionex Acclaim 300 C18 C A Waters Xterra MS C8 C A Bischoff ProntoSIL 300-5-C18 H C A Phenomenex Luna C5 C A Waters Delta-Pak C18 300A C A Waters MicroBondapak C18 AC Bischoff ProntoSIL HyperSORB 120 ODS AC Agilent Zorbax StableBond 80A C8 CA Thermo/ hypersil hypersil PAH C18 AC Agilent Zorbax Rx-C8 CA Bischoff ProntoSIL 120-5-C18 Aqplus C A Grace (Alltech) Platinum C18 CA hplc columns | introduction Phenomenex Jupiter 300 C18 CA Grace VisionHT C18 A.

9 C Varian Polaris C18-Ether CA Phenomenex Luna Phenyl-Hexyl AC Waters Atlantis T3 C18 AC Grace (Alltech) Alltima C18-LL C A Tosoh TSKgel 80Ts QA C A Bischoff ProntoSIL 120-5-C18 ace-EPS AC Grace (Alltech) Alltima C18-WP C A Grace (Vydac) Vydac 218MS C18 AC Waters YMC Hydrosphere C18 C A Waters Acquity UPLC BEH phenyl CA Grace (Alltech) Brava BDS C18 C A Waters Spherisorb C8 AC Bischoff ProntoSIL 60-5 C8 SH CA YMC J'Sphere L80 C18 CA Varian Polaris C18-A C A Bischoff ProntoSIL 300-55-C18 ace-EPS A C YMC J'Sphere M80 C18 C A Dionex Acclaim Organic Acid C18 C. A Agilent Zorbax Eclipse XDB-C8 C A Waters Xterra C18 RP CA Waters Atlantis dC18 b C A Grace (Alltech) Alltima C8 CA Supelco Ascentis Express C8 C A Whatman Partisil C8 CA Thermo hypersil GOLD aQ.

10 CA Merck LiChrospher 60 RP-Select B C18.. AC Phenomenex Selectosil C18 C A Bischoff ProntoSIL 120-5 C8 SH C A Merck LiChrosorb RP-18 C A Grace (Alltech) Allsphere ODS1 C A Grace (Vydac) Vydac 218TP C18 C A Waters Symmetry Shield C8 AC Waters Acquity UPLC BEH Shield RP18 EP C A Thermo/ hypersil Hypurity C4 CA Macherey Nagel Nucleosil C18 CA MacMod/ACT ACE 5 C4-300 CA Agilent Zorbax StableBond 300A C18 C A Varian Polaris C8-Ether C. A Grace (Alltech) Prosphere C18 300 AC Bischoff ProntoSIL 60-5-Phenyl A C Grace (Vydac) Vydac 201TP C18.. C A Waters Nova-Pak Phenyl.


Related search queries