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研究室での画像処理 ImageJの使い方・基礎編
有名なバリアントとしてFijiがある。サイエンス分野で よく使うプラグインをまとめたもので使い易い(この資料 でImage Jを説明しているのは、単純に筆者は説明できる ほどFijiを使っていないため)。Fiji: https://fiji.sc 新しいバリアントの一つに、ImageJFXがある。
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タンパク定量 (BCA法)
www.iqb.u-tokyo.ac.jp一段階のBiuret反応でできるCu(I)とペプチドの錯体自体にも吸光はありますが、測定に使うには弱すぎます。 なので、二段階目のBCAとの錯体形成反応で562nmに吸収極大を持つより吸光度の高い錯体へと変換していま す。
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