Liquid Chromatography - SUPELCO

1 LiquidChromatographySUPELCO110 Order: Technical Service: Web: ApplicationsAmino Acids, CarbohydratesAmino Acids, Dabsyl (HPLC)Column: SUPELCOSIL LC-DABS, 15cm x ID, 3 m particlesCat. No.: 59137 Mobile Phase: A = KH2PO4, pH with potassium hydroxideB = acetonitrile:methanol (70:30)Flow Rate: : VIS, 436nmInj.: 5 L, 50pmole each amino acid on-columnCarbohydrates (HPLC)Column: SUPELCOGEL Ca, 30cm x IDCat. No.: 59305-UMobile Phase: waterFlow Rate: : 80 CDet.: RIInj.: 10 L, each analyte in waterDark Corn Syrup (HPLC)Column: SUPELCOGEL Ag, 30cm x IDCat. No.: 59315 Mobile Phase: waterFlow Rate: : 85 CDet.: RIInj.: 10 L of 1g syrup/10mL DI water, filtered ( m filter) -Cyclodextrin, Hydrolyzed (HPLC)Column:Two TSKgel G-Oligo-PW, 30cm x ID, 6 m particlesCat.

2 No.:808031 Mobile Phase:distilled waterFlow Rate: 1mL/minTemp.:60 CDet.:refractive indexGradient ProgramTime (min)%B020420925232524323032374046605475 59756020712-01351. o-Phospho-serine2. o-Phospho-threonine3. L-Aspartic acid4. o-Phospho-tyrosine5. Cysteic acid6. L-Glutamic acid7. Carboxymethylcysteine8. S-Sulfocysteine9. L-Asparagine10. L-Glutamine11. L-Serine12. Methionine sulfoxide13. L-Threonine14. Glycine15. L-Alanine16. L-Arginine17. Methionine sulfone18. L-Proline19. L-Valine20. L-Methionine21. L-Isoleucine22. L-Leucine23. L-Tryptophan24. L-Phenylalanine25. Ammonia26. L-Cystine27. L-Lysine28. L-Histidine29. L-Tyrosine010 2030405023456789101314151216171920211822 232428292725 Min26111795-0016123456780510202515 Min1.

3 Maltotriose2. Maltose3. Glucose4. Xylose5. Arabinose6. Ribitol7. Arabitol8. Xylitol794-0029156432051015 Min1. DP62. DP53. DP44. DP35. DP26. Glucose713-09171520 MinDP=1DP=6DP=4DP=2DP=3DP=7DP=5 LiquidChromatographySUPELCO111 Order: Technical Service: Web: ApplicationsCarbohydrates, DrugsChito Oligosaccharides (HPLC)Column: TSKgel G-Oligo-PW, 30cm x ID, 6 m particlesCat. No.: 808031 Eluent: distilled waterFlow Rate: : 60 CDet.: RICarbohydrates (HPLC)Column: SUPELCOSIL LC-NH2, 25cm x ID, 5 m particlesCat. No.: 58338 Mobile Phase: acetonitrile:water (75:25)Flow Rate: 2mL/minDet.: refractive indexInj.: 10 L, 150 g each sugarAlkaloids (HPLC)Column: Discovery RP-AmideC16, 15cm x ID, 5 m particlesCat.

4 No.: 505013 Mobile Phase: methanol:25mM KH2PO4, pH (20:80)Flow Rate: 2mL/minTemp.: 35 CDet.: UV, 254nmInj.:10 L Allegra Ingredients (HPLC)Column: Discovery Cyano, 15cm x ID, 5 m particlesCat. No.: 59356-UMobile Phase: acetonitrile:25mM KH2PO4, pH (15:85)Flow Rate: 1mL/minTemp.: 25 CDet.: UV, 254nm794-0325123451. Chitohexose2. Chitopentose3. Chitotetrose4. Chitotriose5. Chitobiose1520 Min713-0908324504812 Min11. Fructose2. Glucose3. Sucrose4. Maltose5. LactoseG0002351. Codeine2. Strychnine3. Quinidine4. Quinine5. Noscapine6. Papaverine024681012 Min1234560246810 Min1. Methanol2. Pseudoephedrine3. FexofenadineG001279123 LiquidChromatographySUPELCO112 Order: Technical Service: Web: ApplicationsDrugsAnalgesics (HPLC)Column: SUPELCOSIL LC-ABZ, 5cm x ID, 5 m particlesCat.

5 No.: 59141 Mobile Phase: acetonitrile:25mM KH2PO4,pH with phosphoricacid (20:80)Flow Rate: 2mL/minDet.: UV, 230nmInj.: 5 L mobile phase, analyte quantities shownAnalgesics and Antihistamines in Dosage Form (HPLC)Column: Discovery RP-AmideC16, 15cm x ID, 5 m particlesCat. No.: 505013 Mobile Phase: A = acetonitrileB = 25mM KH2PO4, pH Rate: 2mL/minTemp.: ambientDet.: UV, 214nmInj.:10 LAmides (HPLC)Column: Discovery RP-AmideC16, 15cm x ID, 5 m particlesCat. No.: 505013 Mobile Phase: 80:20 25mM Potassium Phosphate (pH ):MeOHFlow Rate: : 35 CDet.: UV at 254nmInj.:10 LAnalgesic Drugs (HPLC)Columns: 15cm x ID, 5 m particlesCat. Nos.: 567516-U (HS F5), 504955 (C18)Mobile Phase: (A) 10mM ammonium acetate, formic acid; (B) MeOHFlow Rate: : 35 CDet.

6 : UV at 254nmInj.:10 L713-017802 MinDextromethorphan(100 g/mL)Acetylsalicylic acid(50 g/mL)G0007850468102 Min2341. Doxylamine2. Pseudoephedrine3. Codeine4. Acetaminophen5. Chlorpheniramine51 Gradient ProgramTime (min)%B09029067010700246810121416 Min1234561. Codeine2. Strychnine3. Quinidine4. Quinine5. Noscapine6. PapaverineG0006911. p-Aminophenol (100 g/mL)2. Acetaminophen (10 g/mL)3. Acetanilide (10 g/mL)4. Phenacetin (10 g/mL)02468101214 Min02468101214 MinDiscovery C18:Elutes at void volume Gradient ProgramTime (min)%A%B0901039010105050155050 43124312G001527G001526 Discovery HS F5:Good RetentionLiquidChromatographySUPELCO113 Order: Technical Service: Web: ApplicationsDrugsAniline Homologs (HPLC)Coumn: Discovery C18, 15cm x ID, 5 m particlesCat.

7 No.: 504955 Mobile Phase: MeOH:H2O (60:40)Flow Rate: 1mL/minTemp.: 30 CDet.: UV, 254nmAntibiotics (Fluoroquinolones from Tablets) (HPLC)Columns: 15cm x ID, 5 m particlesCat. Nos.: 504955 (C18), 568522-U (HS C18)Mobile Phase: (A) 25 mM potassium phosphate (pH )(B) CH3 CNFlow Rate: : 35 CDet.: UV, 220nmInj.:10 LAnticonvulsants (HPLC)Column: Discovery C18, 15cm x ID, 5 m particlesCat. No.: 504955 Mobile Phase: acetonitrile:25mM KH2PO4, (40:60)Flow Rate: 1mL/minTemp.: 40 CDet.: UV, 254nmInj.:10 L024681012141618 Min1234561. Aniline2. N-Ethylaniline3. N-Propylaniline4. N,N-Dimethylaniline5. N-Butylaniline6. N,N-DiethylanilineG00029502468101214 Min12345602468101214 Min1234561. Levofloxacin2.

8 Ciprofloxacin3. Lomefloxacin4. Sparfloxacin5. Grepafloxacin6. TrovafloxacinDiscovery HS C18 Discovery C18G001425G001424 Gradient ProgramTime (min)%A%B090102565351. Clonazepam2. Clorazepate3. DiazepamG0001880246810 Min123 LiquidChromatographySUPELCO114 Order: Technical Service: Web: ApplicationsDrugsAntihypertensive-Diuret ic Combination (HPLC)Column: Discovery RP-AmideC16, 15cm x ID, 5 m particlesCat. No.: 505013 Mobile Phase: methanol: 25mM K2 HPO4, pH (22:78)Flow Rate: 1mL/minTemp.: 30 CDet.: UV, 254nmInj.:10 LAnticonvulsants (HPLC)Columns: 15cm x ID, 5 m particlesCat. Nos.: 504955 (C18), 59353-U (C8)Mobile Phase: 70:30 water:CH3 CNFlow Rate: : UV, 254nmTemp.: 20 CInj.:10 LAntihistamines (HPLC)Column: Discovery C8, 15cm x ID, 5 m particlesCat.

9 No.: 59353-UMobile Phase: acetonitrile:25mM KH2PO4, pH (8:92)Flow Rate: 2mL/minTemp.: 25 CDet.: UV, 254nmInj.: 2 LAntihypertensive ACE Inhibitors (HPLC)Column: Discovery RP-AmideC16, 15cm x ID, 5 m particlesCat. No.: 505013 Mobile Phase: acetonitrile:25mM KH2PO4, pH (33:67)Flow Rate: : 35 CDet.: UV, 214nmInj.: 3 L1. Atenolol2. Amiloride3. Hydrochlorothiazide4. CaffeineG0002800246810 Min1234024681012141618 Min1230 2 4 6 8 1012141618 Min1231. Clonazepam2. Clorazepate3. DiazepamG000748G000746G0012781. Maleic acid2. Doxylamine3. Chlorpheniramine4. Carbinoxamine5. Brompheniramine024681012141618 Min132451. Enalapril2. Lisinopril3. CaptoprilG000279 Discovery C8 Discovery C18 LiquidChromatographySUPELCO115 Order: Technical Service: Web: ApplicationsDrugsAnti-Inflammatory Drugs(Aminopryridine and Piroxicam) (HPLC)Columns: Discovery C18, 15cm x ID, 5 m particlesCat.

10 Nos.: 504955 Mobile Phase: A = 85:15, 10 mM potassium phosphate (pH ): CH3 CNB = 85:15, 10 mM potassium phosphate (pH ): CH3 CNFlow Rate: : UV, 220nmInj.: 5 LAnti-Inflammatory Drugs and Diuretics in Serum (HPLC)Column: Hisep, 15cm x ID, 5 m particlesCat. No.: 58935 Mobile Phase: A = acetonitrile, B = 180mM ammonium acetate, pH Rate: 2mL/minTemp.: 30 CDet.: UV, 267nmInj.: 10 L horse serum containing 5 g/mL hydrochlorothiazide,10 g/mL each other drugAntihypertensive Drugs (HPLC)(Beta/Calcium Channel Blockers and ACE Inhibitor)Column: Discovery RP-AmideC16, 15cm x ID, 5 m particlesCat. No.: 505013 Mobile Phase: methanol:25mM KH2PO4, pH (40:60)Flow Rate: 1mL/minTemp.: 35 CDet.: UV, 214nmInj.