MS Columns

1 MS Columns MS Columns Order no. 130-042-201. MS Columns plus tubes Order no. 130-122-727. Contents Columns are flow stop and do not run dry. 1. Description Void volume: 60 L. Reservoir volume: mL. Background information Typical flow rate for PBS (phosphate-buffered saline). containing BSA (bovine serum albumin): mL/. Technical specifications min. Applications MS Columns are for single use only. Reagent and instrument requirements Applications 2. Use of MS Columns MS Columns have been developed for positive selection of human Preparation of MS Columns and animal cells, especially rare cells, out of a heterogeneous cell Magnetic separation using MS Columns suspension in combination with a MACS Separator.

2 MS Columns can also be used for depletion of cells which strongly express the magnetically labeled surface antigen. They can also be used to separate other biological material such as plant cells, bacteria, 1. Description viruses, protozoa, cell organelles, etc. This product is for research use only. Do not use MS Columns in combination with magnetic particles Components MS Columns (# 130-042-201): other than MACS MicroBeads. Magnetic forces in the column are 25 MS Columns and plungers, sterile packed. very high and may damage biological material if other beads are used. or MS Columns are not suitable for particles larger than 30 m.

3 MS Columns plus tubes (# 130-122-727): To remove clumps and to prevent aggregates in the sample, 25 MS Columns and plungers (# 130-042-201), resuspend material carefully and pass through 30 m nylon mesh sterile packed, and 75 5 mL Tubes for (Pre-Separation Filters (30 m), # 130-041-407) before separation. MS Columns (# 130-091-598), sterile packed as Samples or buffers with high viscosity might cause reduced 3 25 tubes. column flow or column clogging. Storage Store Columns dry at 10 35 C and protected from light. The expiration date is indicated on Reagent and instrument requirements the box label. Do not use after this date.

4 Buffer: Prepare a solution containing phosphate-buffered Background information saline (PBS), pH , bovine serum albumin (BSA), and 2 mM EDTA by diluting MACS BSA Stock Solution The patented MACS Column Technology is based on the use of (# 130 091 376) 1:20 with autoMACS Rinsing Solution MACS MicroBeads, MACS Columns , and MACS Separators. (# 130 091 222). Keep buffer cold (2 8 C). Degas buffer before MS Columns have been developed for the gentle isolation of use, as air bubbles could block the column. MicroBead-labeled cells. As MACS MicroBeads are extremely Note: The recommended buffer is PBS supplemented with EDTA and BSA.

5 The small, superparamagnetic particles, a high-gradient magnetic suitability of other buffers has to be tested experimentally. field is required to retain the labeled cells. MS Columns contain Note: Use degassed buffer only! Degas buffer by applying vacuum, an optimized matrix to generate this strong magnetic field when preferentially with buffer at room temperature. Excessive gas in running buffer placed in a permanent magnet such as the MiniMACS Separator, will form bubbles in the matrix during separation. This may lead to clogging of OctoMACS Separator, or SuperMACS II Separator. the column and decrease the quality of separation.

6 MACS MicroBeads for magnetic labeling of cells. Technical specifications MiniMACS Separator (# 130-042-102), OctoMACS. Column capacity: 1 10 magnetically labeled cells from up to Separator (# 130-042-109), or SuperMACS II Separator 2 10 total cells. (# 130-044-104). Note: Column capacity may decrease when separating cells larger than lymphocytes. MACS MultiStand (# 130-042-303) in combination with MiniMACS Separator or OctoMACS Separator. Recommended sample size for leukocytes: 10 10 labeled cells in 10 2 10 total cells. Adapter for MS, LS, and LD Columns for use with SuperMACS. II Separator. Typical enrichment rate: 50-fold to up to 1,000-fold, depending on the strength and specificity of the magnetic labeling.

7 Up to MACS Acrylic Tube Rack (# 130-041-406) or OctoMACS. 10,000fold enrichment can be achieved by separation over two Acrylic Tube Rack (# 130-090-448). sequential Columns . (Optional) Pre-Separation Filters (30 m) (# 130-041-407) to remove cell clumps. Miltenyi Biotec & Co. KG. Friedrich-Ebert-Stra e 68, 51429 Bergisch Gladbach, Germany Phone +49 2204 8306-0, Fax +49 2204 85197. page 1/2. Order no. 130-042-201. Order no. 130-122-727. 2. Use of MS Columns 4. Remove MS Column from the separator and place it on a new collection tube. Preparation of MS Columns 1. Insert MS Column with the column wings to the front into a 5.

8 Pipette 1 mL buffer onto the MS Column. Immediately flush MACS Separator according to A) or B). out fraction with the magnetically labeled cells by firmly applying the plunger supplied with the column. A) Use with MiniMACS or OctoMACS Separator 6. (Optional) To increase the purity of the magnetically labeled fraction, the eluted fraction can be enriched over a second MS Column. Repeat the magnetic separation procedure as described in steps 2 to 5 by using a new column. Refer to for all data sheets and protocols. Miltenyi Biotec provides technical support worldwide. Visit to find your nearest Miltenyi Biotec contact. Legal notices Limited product warranty Miltenyi Biotec & Co.

9 KG and/or its affiliate(s) warrant this product to be free from material defects in workmanship and materials and to conform substantially Attach the MiniMACS Separator or OctoMACS Separator to with Miltenyi Biotec's published specifications for the product at the time of order, the MACS MultiStand and place MS Column in the Separator. under normal use and conditions in accordance with its applicable documentation, for a period beginning on the date of delivery of the product by Miltenyi Biotec or its Place a collection tube under the MS Column. authorized distributor and ending on the expiration date of the product's applicable shelf life stated on the product label, packaging or documentation (as applicable) or, B) Use with SuperMACS II Separator in the absence thereof, ONE (1) YEAR from date of delivery ( Product Warranty ).

10 Miltenyi Biotec's Product Warranty is provided subject to the warranty terms as set For use of MS Columns with the SuperMACS II Separator forth in Miltenyi Biotec's General Terms and Conditions for the Sale of Products please refer to the respective data sheet. and Services available on Miltenyi Biotec's website at , as in effect at the time of order ( Product Warranty ). Additional terms may apply. BY. 2. Prepare MS Column by rinsing with buffer: apply 500 L of USE OF THIS PRODUCT, THE CUSTOMER AGREES TO BE BOUND BY THESE. degassed buffer on top of the column and let the buffer run TERMS. through. MS Columns are flow stop and do not run dry.