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Phase I/II clinical trial of everolimus combined with ...
USA) on a BioRad QX200 Droplet Digital PCR System. Each assay run included a mutation-positive template and no-template controls. The fractional abundance (%) of the PIK3CA mutation was calculated from the number of FAM-positive events (mutation-positive)
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Research Paper Detecting Circulating Tumor DNA in ...
www.jcancer.orgThe QX200 droplet digital PCR system (Bio-Rad, Hercules, USA) was used for mutant detection according to the manufacturer’s instructions. The ddPCR reactions were carried out in 20 µl volumes using 10 µl 2x ddPCR SuperMix (Bio-Rad), 5 µl cfDNA template, µl (20x) wild type probe/primer 1 ...
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