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Procedure & Checklist – Preparing Multiplexed Microbial ...
Procedure & Checklist – Preparing Multiplexed Microbial Libraries Using SMRTbell® Express Template Prep Kit 2.0. Before You Begin . This procedure describes how to prepare multiplexed SMRTbell libraries for sequencing on the Sequel®, Sequel II, and Sequel IIe Systems. For each microbe, 1 µg of gDNA is required. For optimal
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www.pacb.comPage 3 Powering Up the Instrument To power up the instrument, press the green illuminated button located on the right side panel of the instrument.
Extracting DNA Using Phenol-Chloroform - PacBio
www.pacb.comExtracting DNA Using Phenol-Chloroform . Reagents Needed . 1. Phenol/chloroform/isoamyl a lcohol (PCI) solution (25:24:1) DNase (RNase- and Protease-Free - Molecular Biology grade), pH 7.8-8.2 Important: acidic pH makes the DNA go into the phenolic layer while RNA goes into the aqueous layer Suppliers
Procedure & Checklist – Using AMPure PB Beads for Size ...
www.pacb.comconcentration of the SMRTbell library to be size selected must be 0.5–10 ng/µL. Use of higher concentrations (>15-100 ng/µL) decreases the efficiency of reduction of short insert SMRTbell templates. Adjust the sample concentration so that the DNA concentration is within this range. The volume of diluted AMPure PB beads to
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