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RT-qPCR guidelines - University of California, Davis
Detection method:-Sybr Green I-Probes; TaqMan, Beacons, etc-Background correction-Fit point method-Other models (Log)-Singleplex, multiplex Quantification strategy:-Absolute quantification * Using calibration curve-Relative quantification * Normalization with RG * Normalization with more (>3) RGs Test method:-SAS, SPSS, Excel-Normality of data ...
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