System Manual and Quick Reference - Agilent

1 Agilent TechnologiesAgilent 1290 Infinity II LCSystem Manual and Quick ReferenceAgilent 1290 Infinity II LC System Manual and Quick ReferenceNotices Agilent Technologies, Inc. 2014-2018No part of this Manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla-tion into a foreign language) without prior agreement and written consent from Agi-lent Technologies, Inc. as governed by United States and international copyright Part NumberG7104-90300 Rev. DEdition12/2018 Printed in GermanyAgilent TechnologiesHewlett-Packard-Strasse 8 76337 WaldbronnWarrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions.

2 Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this Manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or perfor-mance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the sep-arate agreement shall Licenses The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such Rights LegendIf software is for use in the performance of a Government prime contract or sub-contract, Software is delivered and licensed as Commercial computer software as defined in DFAR (June 1995), or as a commercial item as defined in FAR (a)

3 Or as Restricted computer software as defined in FAR (June 1987) or any equivalent agency regu-lation or contract clause. Use, duplication or disclosure of Software is subject to Agi-lent Technologies standard commercial license terms, and non-DOD Departments and Agencies of the Government will receive no greater than Restricted Rights as defined in FAR (c)(1-2) (June 1987). Government users will receive no greater than Limited Rights as defined in FAR (June 1987) or DFAR (b)(2) (November 1995), as applicable in any technical NoticesCAUTIONA CAUTION notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly per-formed or adhered to, could result in damage to the product or loss of important data.

4 Do not proceed beyond a CAUTION notice until the indicated condi-tions are fully understood and met. WARNINGA WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a WARNING notice until the indi-cated conditions are fully understood and 1290 Infinity II LC System Manual and Quick Reference3In This BookIn This BookThis book describes the Agilent 1290 Infinity II chapter gives an introduction to the Agilent 1290 Infinity II LC, the underlying concepts and the features of the Agilent 1290 Infinity II Configuration SettingsThis chapter describes how to configure the Start GuideThis chapter provides information on running an Agilent 1290 Infinity II LC.

5 4 Parts and ConsumablesThis chapter provides information on additional parts and consumables. 5 AppendixThis chapter provides addition information on safety, legal, and 1290 Infinity II LC System Manual and Quick ReferenceContentsContents1 Introduction 7 Product Description8 Features of the Agilent 1290 Infinity II LC9 System Components10 Solutions18 Optimizing the Stack Configuration20 Standard Capillary Setup26 Leak and Waste Handling272 Configuration Settings33 General Information on LAN Configuration34 Instrument Configuration35 Lab Advisor373 Quick Start Guide 43 Best Practices44 Prepare a Run49 Check Out the System564 Parts and Consumables67 Tool Kit G7120-6870868 InfinityLab Quick Connect and Quick Turn Fittings69 Safety Caps and Solvent Bottles73 InfinityLab Flex Bench Family77 Agilent 1290

6 Infinity II LC System Manual and Quick Reference5 Contents5 Appendix79 Safety80 Waste Electrical and Electronic Equipment Directive86 Lithium Batteries Information87 Radio Interference88 Sound Emission89 Solvent Information90UV Radiation91 Declaration of Conformity for HOX2 Filter92 Agilent Technologies on Internet936 Agilent 1290 Infinity II LC System Manual and Quick ReferenceContents7 Agilent 1290 Infinity II LC System Manual and Quick ReferenceAgilent Technologies1 IntroductionProduct Description8 Features of the Agilent 1290 Infinity II LC9 System Components10 Flexible Pump11 High Speed Pump12 Multisampler13 Vialsampler14 Multicolumn Thermostat15 Diode Array Detector (DAD)16 Variable Wavelength Detector (VWD)

7 17 Solutions182D-LC Solution18 High Dynamic Range DAD Solution18 Method Transfer Solution19 Optimizing the Stack Configuration20 Agilent InfinityLab Flex Bench20 One Stack Configuration22 Two Stack Configuration25 Standard Capillary Setup26 Leak and Waste Handling27 Waste Guidance31 Waste Concept32 This chapter gives an introduction to the Agilent 1290 Infinity II LC, the underlying concepts and the features of the Agilent 1290 Infinity II 1290 Infinity II LC System Manual and Quick Reference1 IntroductionProduct DescriptionProduct DescriptionThe Agilent 1290 Infinity II LC System has a high reliability and robustness, and improves analytical, instrument, and laboratory separation and detection performance delivers analysis data of high quality - the perfect front-end for high-end LC/MS throughput for any application is achieved through high sample capacity and fast injection cycles, combined with new levels of , seamless integration in existing infrastructure and smooth method transfer from legacy equipment enable non-disruptive transition to highest productivity and lowest cost of 1290 Infinity II LC System Manual and Quick Reference9 Introduction1 Features of the Agilent 1290 Infinity II LCFeatures of the Agilent 1290 Infinity II LC More chromatographic resolution.

8 Specially designed components in the sample flow path achieve low System dispersion. Higher peak capacity for challenging separations: switch easily between single dimension UHPLC and 2D-LC. Lower carryover for uncompromised data quality: multiwash capabilities of the 1290 Infinity II Multisampler reduce carryover to less than 10 ppm even for challenging compounds such as chlorohexidine. Unique detection capabilities: combine low detection limits with an ultra-wide dynamic range by using the new 1290 Infinity II HDR-DAD or 1290 Infinity II ELSD. Faster injection cycles with dual needle injection for higher sample throughput. Higher sample capacity per bench space: up to 6144 samples within the footprint of a standard Agilent stack.

9 Significantly better usability: dead-volume-free UHPLC fluidic connections can be easily achieved with the new InfinityLab Quick Connect fittings. flexibility for all applications: due to the wide power, temperature and automatically scalable injection range, gradient options and intelligent System emulation technology. Seamless transfer of methods between LCs, regardless of the brand facilitated by Intelligent System Emulation Technology (ISET) delivering unchanged retention time and peak resolution. Seamless integration in your chromatography data System : Agilent s Instrument Control Framework (ICF) enables smooth control of Agilent LC instrumentation through third-party chromatography data 1290 Infinity II LC System Manual and Quick Reference1 IntroductionSystem ComponentsSystem ComponentsThe Infinity II LC System can be set up with two different types of pumps.

10 With the Flexible Pump, combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump, or with the High Speed Pump, allowing you to run fast gradients for high laboratory Infinity II LC System consists of the following components: High Speed Pump or Flexible Pump Multisampler or Vialsampler Multicolumn Thermostat Diode Array Detector or Variable Wavelength Detector Solvent CabinetThe Agilent 1290 Infinity II LC is described in more detail in the following sections. All modules are stackable, see One Stack Configuration on page 22 and Two Stack Configuration on page specifications, please refer to the individual module user 1290 Infinity II LC System Manual and Quick Reference11 Introduction1 System ComponentsFlexible PumpThe Agilent 1290 Infinity II Flexible Pump improves your efficiency by combining the performance of a high-pressure mixing UHPLC pump with the flexibility of a low-pressure mixing UHPLC pump.