TEST SOLUTIONS (TS) - 药物在线-快捷药物信息平台

1 First Supplement to USP 35 NF 30 SOLUTIONS / Test Solutions5321any necessary correction. Each mL of N sodium thiosul-Acid Ferric Chloride TS Mix 60 mL of glacial aceticfate is equivalent to mg of CuSO4 5H2O. Adjust theacid with 5 mL of sulfuric acid, add 1 mL of ferric chloridefinal volume of the solution by the addition of enough ofTS, mix, and mixture of hydrochloric acid and water so that each mLAcid Ferrous Sulfate TS See Ferrous Sulfate, Acid, mg of CuSO4 Stannous Chloride TS See Stannous Chloride, Acid,Ferric Chloride CS Dissolve about 55g of ferric chlorideTS.(FeCl3 6H2O) in enough of a mixture of 25 mL of hydro-Acid Stannous Chloride TS, Stronger See Stannouschloric acid and 975 mL of water to make 1000 mL. PipetChloride, Acid, mL of this solution into a 250-mL iodine flask, add 15 Albumen TS Carefully separate the white from the yolkmL of water, 3g of potassium iodide, and 5 mL of hydro-of a strictly fresh hen s egg.

2 Shake the white with 100 mL ofchloric acid, and allow the mixture to stand for 15 until mixed and all but the chalaza has undergoneDilute with 100 mL of water, and titrate the liberated iodinesolution; then filter. Prepare the solution N sodium thiosulfate VS, adding 3 mL of starch TSAlcohol Phenol TS Dissolve 780 mg of phenol in alco-as the indicator. Perform a blank determination with thehol to make 100 quantities of the same reagents, and make any neces-Alcoholic TS It contains 95 parts of speciallysary correction. Each mL of N sodium thiosulfate isdenaturated alcohol 3A with 5 parts of isopropyl to mg of FeCl3 6H2O. Adjust the final vol-The final concentrations are approximately 90% alcohol, 5%ume of the solution by the addition of enough of the mix-methanol, and 5% isopropanol. ture of hydrochloric acid and water so that each mL con-[NOTE A suitable grade is available as Reagent alcohol,tains mg of FeCl3 number R8382, available at ]Alcoholic Ammonia TS See Ammonia TS, SOLUTIONSA lcoholic Mercuric Bromide TS See Mercuric BromideTS, TEST Potassium Hydroxide TS See Potassium Hy-droxide TS, Cupric Citrate TS See Cupric Citrate TS,TEST SOLUTIONS (TS) Cupric Citrate TS 2 See Cupric Citrate TS 2,Certain of the following test SOLUTIONS are intended for acid-base indicators in volumetric analyses.

3 Such solutionsAlkaline Cupric Iodide TS See Cupric Iodide TS, be so adjusted that when mL of the indicatorAlkaline Cupric Tartrate TS (Fehling s Solution) See Cu-solution is added to 25 mL of carbon dioxide-free water,pric Tartrate TS, mL of N acid or alkali, respectively, will produceAlkaline Mercuric Potassium Iodide TS Seethe characteristic color change. Similar SOLUTIONS are in-Mercuric Potassium Iodide TS, for use in pH measurement. Where no special direc-Alkaline Picrate TS See Picrate TS, for their preparation are given, the same solution isAlkaline Sodium Hydrosulfite TS See Sodium Hydrosul-suitable for both TS, it is directed that a volumetric solution be used asAmaranth TS Dissolve 20 mg of amaranth in 10 mL ofthe test solution, standardization of the solution used as not Acid TS Accurately weigh 5gIn general, the directive to prepare a solution fresh indi-of sodium sulfite, g of sodium bisulfite, and 700 mg ofcates that the solution is of limited stability and must be1,2,4-aminonaphtholsulfonic acid, and mix.

4 Prepare ami-prepared on the day of acid TS fresh on the day of use by dis-For the preparation of Test SOLUTIONS , use reagents of thesolving g of the dry mixture in 10 mL of described under TS It contains between and ofAcetaldehyde TS Mix 4 mL of acetaldehyde, 3 mL ofNH3. Prepare by diluting 350 mL of Ammonia Water,alcohol, and 1 mL of water. Prepare this solution (see in the section, Reagents) with water to makeAcetate Buffer TS Dissolve 320g of ammonium acetate1000 500 mL of water, add 5 mL of glacial acetic acid, diluteAmmonia TS 2 Prepare by diluting mL of Ammo-with water to mL, and mix. This solution has a pHnia Water, Stronger (see Reagent Specifications in the sectionbetween and ) with water to make 100 Acid, Glacial, TS Determine the water contentAmmonia TS, Alcoholic A solution of ammonia gas inof a specimen of glacial acetic acid by the Titrimetric Methodalcohol. Clear, colorless liquid having a strong odor of am-(see Water Determination 921 ).

5 If the acid contains moremonia. Specific gravity: about It contains between 9%than of water, add a few mL of acetic anhydride, mix,and 11% of NH3. Store it in alkali-resistant containers, in aallow to stand overnight, and again determine the watercold If the acid contains less than of water, addAmmonia TS, Stronger Use Ammonia Water, Strongersufficient water to make the final concentration between(see in the section Reagents). and , mix, allow to stand overnight, and againAmmonia Ammonium Chloride Buffer TS Dissolvedetermine the water content. Repeat the adjustment g of ammonium chloride in water, add 570 mL ofacetic anhydride or water, as necessary, until the resultingammonium hydroxide, and dilute with water to 1000 shows a water content of not more than Cyanide TS Dissolve 2g of potassium cya-Acetic Acid, Strong, TS Add mL of glacial aceticnide in 15 mL of ammonium hydroxide, and dilute withacid, and dilute with water to 1000 mL.

6 This solution con-water to 100 about 30% (v/v) of CH3 COOH and has a concentrationAmmoniacal Potassium Ferricyanide TS Dissolve 2gof about potassium ferricyanide in 75 mL of water, add 25 mL ofAcetic Acid Ammonium Acetate Buffer TS Dissolveammonium hydroxide, and g of ammonium acetate in water, add 57 mL of glacialAmmoniated Cupric Oxide TS See Cupric Oxide, Am-acetic acid, and dilute with water to 1000 , , Buffered, TS Dissolve g of sodium ace-Ammonium Acetate TS Dissolve 10g of ammoniumtate and 42g of sodium chloride in about 100 mL of water,acetate in water to make 100 add 68 mL of N hydrochloric acid and 150 mL ofAmmonium Carbonate TS Dissolve 20g of ammoniumacetone. Mix, and dilute with water to 500 and 20 mL of ammonia TS in water to make from August 1, 2012 Copyright (c) 2012 The United States Pharmacopeial Convention. All rights from by nEwp0rt1 on Tue Jun 12 01:19:55 EDT 20125322 Test SOLUTIONS / SolutionsFirst Supplement to USP 35 NF 30 Ammonium Carbonate TS 2 Prepare a 158-mg/mL so-100 mL of cold water in a glass-stoppered bottle, the stop-lution of ammonium carbonate in of which should be lubricated with petrolatum.

7 Store itAmmonium Chloride TS Dissolve g of ammoniumin a cold place, protected from in water to make 100 Sodium Acetate TS Dissolve 100g of sodiumAmmonium Chloride Ammonium Hydroxide TS Mixacetate in 1000 mL of glacial acetic acid, add 50 mL ofequal volumes of water and ammonium hydroxide, and sat-bromine, and with ammonium TS Add 8g of p-bromoaniline to aAmmonium Molybdate TS Dissolve g of finelymixture of 380 mL of thiourea-saturated glacial acetic acid,powdered molybdic acid in a mixture of 14 mL of water10 mL of sodium chloride solution (1 in 5), 5 mL of oxalicand mL of ammonium hydroxide. Cool the solution,acid solution (1 in 20), and 5 mL of dibasic sodium phos-and add it slowly, with stirring, to a well-cooled mixture ofphate solution (1 in 10) in a low-actinic glass bottle. Mix,32 mL of nitric acid and 40 mL of water. Allow to stand forand allow to stand overnight before using. Protect from48 hours, and filter through a fine-porosity, sintered-glasslight, and use within 7 This solution deteriorates upon standing and is un-Bromocresol Blue TS Use Bromocresol Green for use if, upon the addition of 2 mL of dibasicBromocresol Green TS Dissolve 50 mg of bromocresolsodium phosphate TS to 5 mL of the solution, an abundantgreen in 100 mL of alcohol, and filter if precipitate does not form at once or after slightBromocresol Green Methyl Red TS Dissolve g ofwarming.

8 Store it in the dark. If a precipitate forms duringbromocresol green and g of methyl red in 180 mL ofstorage, use only the clear , and dilute with water to 200 Oxalate TS Dissolve g of ammoniumBromocresol Purple TS Dissolve 250 mg of bromo-oxalate in water to make 100 purple in 20 mL of N sodium hydroxide, andAmmonium Phosphate, Dibasic, TS (Ammoniumdilute with water to 250 TS) Dissolve 13g of dibasic ammonium phos-Bromophenol Blue TS Dissolve 100 mg of bromophe-phate in water to make 100 blue in 100 mL of diluted alcohol, and filter if Polysulfide TS Yellow liquid, made by sat-Bromothymol Blue TS Dissolve 100 mg ofurating ammonium sulfide TS with blue in 100 mL of diluted alcohol, and filter ifAmmonium Pyrrolidinedithiocarbamate, Saturated, Add about 10g of ammonium pyrrolidinedithiocarba-Buffered Acetone TS See Acetone, Buffered, to a 1000-mL volumetric flask, and dilute with waterCalcium Chloride TS Dissolve g of calcium chlorideto water to make 100 Reineckate TS Shake about 500 mg ofCalcium Hydroxide TS Use Calcium Hydroxide Topicalammonium reineckate with 20 mL of water frequently dur-Solution (USP monograph).

9 Ing 1 hour, and filter. Use within 2 Sulfate TS A saturated solution of calcium sul-fate in Ammonium Nitrate TS Dissolve g of cericChange to read:ammonium nitrate in 10 mL of N nitric acid. Usewithin 3 Sulfide TS nUse ACS reagent-grade Am-Chloral Hydrate TS Dissolve 50g of chloral hydrate inmonium Sulfide (USP35)a mixture of 15 mL of water and 10 mL of Thiocyanate TS Dissolve 8g of ammo-Chlorine TS (Chlorine Water) A saturated solution ofnium thiocyanate in water to make 100 in water. Place the solution in small, completelyAmmonium Vanadate TS Dissolve g of ammoniumfilled, light-resistant containers. Chlorine TS, even when keptvanadate in 500 mL of boiling water, cool, and add 20 mLfrom light and air, is apt to deteriorate. Store it in a cold,of nitric acid. Mix, cool, and add water to make 1 L. Storedark place. For full strength, prepare this solution polyethylene Acid TS Dissolve 50 mg of chromotropicAnthrone TS Within 12 hours of use, rapidly dissolveacid or its disodium salt in 100 mL of 75% sulfuric acid,35 mg of anthrone in a hot mixture of 35 mL of water andwhich may be made by cautiously adding 75 mL of sulfuric65 mL of sulfuric acid.

10 Immediately cool in an ice bath toacid to mL of temperature, and filter through glass wool. Allow theCobalt Uranyl Acetate TS Dissolve, with warming,solution to stand at room temperature for 30 minutes40g of uranyl acetate in a mixture of 30g of glacial aceticbefore and sufficient water to make 500 mL. Similarly, prepareAntimony Trichloride TS Dissolve 20g of antimonya solution containing 200g of cobaltous acetate in a mix-trichloride in chloroform to make 100 mL. Filter if of 30g of glacial acetic acid and sufficient water toBarium Chloride TS Dissolve 12g of barium chloride inmake 500 mL. Mix the two SOLUTIONS while still warm, andwater to make 100 to 20 . Maintain the temperature at 20 for about 2 Barium Hydroxide TS A saturated solution of bariumhours to separate the excess salts from solution, and thenhydroxide in recently boiled water. Prepare the solutionpass through a dry Chloride TS Dissolve 2g of cobaltous chlo-Barium Nitrate TS Dissolve g of barium nitrate inride in 1 mL of hydrochloric acid and sufficient water towater to make 100 100 TS See 2-Naphthol Red TS Dissolve 500 mg of congo red in a mix-Biuret Reagent TS Dissolve g of cupric sulfate andture of 10 mL of alcohol and 90 mL of g of potassium sodium tartrate in 500 mL of water in am-Cresol Purple TS Dissolve g of metacresol pur-1000-mL volumetric flask.