The pH/pI/pKa problems are straightforward if you apply ...

NoteonpH/pI/pKaProblemsforAminoAcidsThep H/pI/pKaproblemsarestraightforwardif youapplytheserules:pI(electrophoresispro blems),like If thepHis lessthanthepI,theaminoacidwillmove towardthenegative electrode. If thepHis greaterthanthepI,theaminoacidwillmove towardthepositive electrode. If thepHequalsthepI,theaminoacidwillnotmove pH/pKaproblems,like If thepHis lessthanthepKaofa group,thepredominantformwillbetheconjuga teacid. IfthepHisgreaterthanthepKaofa group,thepredominantformwillbetheconjuga tebase. If thepHis equalto thepKaofa group, , , lessthanthepI,sothehistidinemoleculesmov e , notethattheside-chainpKais , ,andsincetheaminogroupis ,most(about90%)ofthemoleculesofhistidine willhave electrode? ,a littlelessthan10%ofthehistidinemolecules willhave a protonatedsidechain,givingthema positive electrode,theacid-baseequilibriumforther estofthesamplewillshifttoproducemoreofth epositive formofthehistidine,whichwillthenalsomove towardthenegative +NNHOO MAJOR form of His at pH= (net neutral)H3N+NNHH+OO Minor form of His at pH= (net + charge)pKa = to the negativeelectrode and drags theequilibrium to the

The pH/pI/pKa problems are straightforward if you apply these rules: pI (electrophoresis problems), like 27.2 If the pH is less than the pI, the amino acid will move toward the negative electrode. If the pH is greater than the pI, the amino acid will move toward the positive electrode.

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Transcription of The pH/pI/pKa problems are straightforward if you apply ...

1 NoteonpH/pI/pKaProblemsforAminoAcidsThep H/pI/pKaproblemsarestraightforwardif youapplytheserules:pI(electrophoresispro blems),like If thepHis lessthanthepI,theaminoacidwillmove towardthenegative electrode. If thepHis greaterthanthepI,theaminoacidwillmove towardthepositive electrode. If thepHequalsthepI,theaminoacidwillnotmove pH/pKaproblems,like If thepHis lessthanthepKaofa group,thepredominantformwillbetheconjuga teacid. IfthepHisgreaterthanthepKaofa group,thepredominantformwillbetheconjuga tebase. If thepHis equalto thepKaofa group, , , lessthanthepI,sothehistidinemoleculesmov e , notethattheside-chainpKais , ,andsincetheaminogroupis ,most(about90%)ofthemoleculesofhistidine willhave electrode? ,a littlelessthan10%ofthehistidinemolecules willhave a protonatedsidechain,givingthema positive electrode,theacid-baseequilibriumforther estofthesamplewillshifttoproducemoreofth epositive formofthehistidine,whichwillthenalsomove towardthenegative +NNHOO MAJOR form of His at pH= (net neutral)H3N+NNHH+OO Minor form of His at pH= (net + charge)pKa = to the negativeelectrode and drags theequilibrium to the