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TRANSFECTION - Bowdish
TRANSFECTION Fiona Whelan: Apr 12th 2012: . Bowdish Lab, McMaster University . Hamilton, ON, Canada . www.bowdish.ca . BACKGROUND - Cell transfection is the act of deliberately introducing a novel nucleic acid into cells in a cell culture.
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CULTURE OF HEK 293/293T C - Bowdish
www.bowdish.caCULTURE OF HEK 293/293T CELLS Charles Yin Last Updated: 11 August 2014 Bowdish Lab, McMaster University Hamilton, ON, Canada www.bowdish.ca BACKGROUND - HEK 293/293T cells are derived from human embryonic kidney transformed with adenovirus 5 DNA
MAINTENANCE CULTURE OF THP-1 CELLS - Bowdish
www.bowdish.caMAINTENANCE & CULTURE OF THP-1 CELLS Created/updated by: D. Bowdish Date: July, 2011 . Bowdish Lab, McMaster University . Hamilton, ON, Canada . www.bowdish.ca . BACKGROUND These cells are monocyte-like cell line derived from leukemia from a one year old boy (ATCC#TIB-202)
ISOLATING BONE MARROW-DERIVED MØ S - …
www.bowdish.caISOLATING BONE MARROW-DERIVED MØ’S Created/updated by: Mike Dorrington Date: 04/07/11 . Bowdish Lab, McMaster University . Hamilton, ON, Canada . www.bowdish.ca . BACKGROUND - The bone marrow of the leg bones of mice is a great source of monocyte/macrophage (Mø)
INTESTINAL PERMEABILITY ASSAY FITC-DEXTRAN - Bowdish
www.bowdish.caGavage the mouse with 150 μl of 80 mg/ml FITC dextran (4kDa). Start the timer after gavaging the first mouse, and note the time of the last gavage.
P ROPAGATION C ULTURING OF RAW264.7 C ELLS
www.bowdish.caProtocol All activities with cells should be conducted under a clean, sterile biosafety cabinet (level 2). Do not expose cells to cold media or violent movement as this can cause dislodging or cell death.