US $ Liquid Chromatography - 思必可有限公司

1 LiquidChromatographyOrder: Technical Service: Web: $13 HPLC: Small MoleculesColumn SelectionHPLC Column Selection: Small Molecules (<2000 mw)This chart is meant to be a starting point for choosing an HPLC column. In some cases, more than one column can be used for ananalysis. Although general rules apply, you should first consult the technical literature, official methodology, or our Technical Servicechemists for definitive column choices for peptides, proteins, and other biopolymers, see pages TECHNIQUEANALYTE CLASSCOLUMNGENERAL (pH ).

2 Discovery C18, C8, RP-AmideC16; Discovery Cyano, Discovery HS C18, HS F5, HS PEGGENERAL (pH 2-13)..SUPELCOGEL ODP-50, TPR-100acids, C18, C8, RP-AmideC16acids, amino .. Discovery C18, RP-AmideC16; SUPELCOSIL LC-DABS amines, C18, RP-AmideC16; Discovery Cyano, Discovery HS F5; SUPELCOGEL TPR-100amines, quaternary (pH> ) ..SUPELCOGEL TPR-100, ODP-50chelating C18, C8, RP-AmideC16; SUPELCOGEL TPR-100, ODP-50drugs, C18, C8, RP-AmideC16; Discovery Cyano, Discovery HS F5reverseddrugs in ; Discovery C18, RP-AmideC16, C8; Discovery HS C18, C8, RP-AmideC16; Discovery LC-18-S; Discovery C18, LC-18-T; Discovery C18, RP-AmideC16peptides.

3 Discovery C18, C8, LC-NH2taxol, LC-Ftricyclic LC-PCN; Discovery C8; Discovery Cyanovitamins, water C18, C8, Ag1, Ag2; TSK-GEL Oligo-PWexclusionpolymers/oligomers, TSK-GEL PWXL ionanions, organic (- charge)..SUPELCOSIL SAX1exchangecations, organic (+ charge)..SUPELCOSIL LC-SCXionicion pairingacids, Discovery C18, Discovery HS C18amines, C18, Discovery HS C18ionacids, SUPELCOGEL H, C610 Hexclusioncarbohydrates, SUPELCOGEL C611, K, Pb, Ag1, Ag2, Cyano, LC-NH2-NP, LC-Si, Discovery HS SUPELCOSIL LC-Diolvitamins, fat SUPELCOSIL LC-NH2-NPorganic solublefatty LC-18reversedpolyaromatic LC-PAHphasepolymers, LC-18vitamins, fat C18, C8, RP-AmideC16, SUPELCOSIL LC-8size exclusionpolymers/oligomers, TSK-GEL HHRL iquidChromatographyOrder.

4 Technical Service: Web: $14 HPLC: Small MoleculesDiscovery Reversed-PhasesCharacteristics of Discovery Reversed-PhasesCoveragePore DiameterSurface AreaPhase mole/m2%CEndcappedAngstromsm2/gHS , Different Separations versus C18 While C18 s from different sources can provide significant differences in re-tention and selectivity, these differences are frequently small and not sufficientto produce really valuable, improved separations. The Discovery suite of re-versed-phases is designed to be complimentary to C18 by combining polar functionality with the standard alkyl/hydrophobic phase.

5 The result It s muchmore likely to achieve an improved, valuable separation with a polarfunctionalized reversed-phase than with another for all small molecule HPLC applicationsDesigned to meet the exacting requirements of pharmaceutical analysis andpurification, Discovery columns are also ideal for all other application segmentsrequiring reversed-phase HPLC, including:- food and beverage- agriculture- environmental- consumer products- clinical- industrial chemical- petrochemical- and more.

6 C18RP-AmideC16HS F5 CyanoHS PEGC8 OSiCH3(CH2)17CH3CH3 Comprehensive Suite of Alkyl and Functionalized Phases=OOnOSi(CH2)3 NHCO-(CH2)14CH3CH3CH3 OSiCH3(CH2)17CH3CH3 OSiCH3(CH2)3 CNCH3 OSiCH3(CH2)7CH3CH3 OSiCH3CH3(CH2)3 FFFFFHS C18On the next page,begin to rediscovermethod : Technical Service: Web: $15 HPLC: Small MoleculesRediscover Method Development024681012 Min12340246810 Min1243 Take Advantage of the Discovery Suite of Reversed-PhasesWhether you re developing a separation thatmust be perfect, or you re just not satisfied withyour reversed-phase to Discovery!

7 A suite of HPLC columns featuring function-alized reversed-phases designed to provide dif-ferentiated separations vs. C18 based on uniquecombinations of polar and hydrophobic Discovery suite of reversed-phases en-ables you to optimize your , retention- selectivity- resolution- analysis minimizing method development :Discovery Reversed-PhasesDiscovery RP-AmideC16 Delivers Better Peak Spacing and Faster Analysis!Scientists have long known polar embedded phases such as Discovery RP-AmideC16 provide unique retention and selectivity compared to C18 s.

8 Taking ad-vantage of Discovery RP-AmideC16 s unique retention and selectivity results in abetter separation vs. Unique selectivity - peak reversal of compounds 3 and 4- Better peak spacing - less dead space between peaks 1 and 2- Higher assay throughput - 20% reduction in analysis time- Improved resolution of critical peak pair 3 and 4 Column:15cm x ID, 5 m particlesMobile Phase: 90:10, 25 mM Potassium Phosphate (pH ): MeOHFlow :20 CDet.: UV at 214nmInj.: 10 LG000232G000233 Discovery C18 Discovery RP-AmideC16HS PEGC yanoHS F5C8RP-AmideC16C18HS C18024681012k Butylbenzene (40:60 water:CH3CN)Sample:1.

9 Nizatidine2. Ranitidine3. Famotidine4. CimetidineLiquidChromatographyOrder: Technical Service: Web: $16 Deliver Better Separations in Less TimeUnique retention and selectivityof Discovery HS F5 enables rapiddevelopment of simple impurityassay where C18 fails!Impurity methods requiring retention and reso-lution of vastly differing analytes may not be suit-ably obtained using simple C18-based simply changing the stationary phase themethod development scientist can avoid:- complicated or forbidden gradients- complex mobile phases- long, drawn-out method developmentOn Discovery HS F5, it took just afew hours to develop an excel-lent (2-AMP) is Unretained on C18 Under Mobile PhaseConditions Used to Assay PiroxicamDecreasing the % Acetonitrile Results in Excessive Piroxicam Retentionand 2-AMP is Still UnretainedIncreasing pH to Retains the 2-AMP but Piroxicam Retention is StillExcessiveC18 Fails02468101214 MinColumn:Discovery C18 15cm x ID, 5 m particlesMobile Phase.

10 45:55, 10 mM Potassium Phosphate (pH ): CH3CN (v/v)Flow : UV at 220nmInj.: 5 LSample: 1. 2-Aminopyridine (10 g/ml in 90:10 buffer:CH3CN)2. Piroxicam (100 g/ml in 90:10 buffer:CH3CN)1. 2-Aminopyridine2. Piroxicam1 202468101214 Min1 2C18 FailsThe Unique Retention and Selectivity of Discovery HS F5 ProducesExcellent Separation at Both pH s024 Min2F5 Delivers Excellent Separation!102468101214 Min1 Same buffer but with lower organic:85:15, 10 mM Potassium Phosphate(pH ): CH3CN (v/v)Same %organic, but changing thepH to : 85:15, 10 mM PotassiumPhosphate (pH ): CH3CN (v/v)85:15, 10 mM Potassium Phosphate(pH ): CH3CN (v/v)85:15, 10 mM Potassium Phosphate(pH ): CH3CN (v/v)SNCONHOHNCH3 OOHPLC: Small MoleculesRediscover Method DevelopmentLiquidChromatographySUPELCO17 Order: Technical Service: Web: X/k C18/k RP-Amide Acetaminophen2.