ELISA technical guide and protocols

← Back to document page

TECH TIP # 65 ELISA technical guide and protocols Table of Contents Introduction ........................................ ........................................ ........................................ ........................................ .................2 Variations between ELISA protocols ........................................ ........................................ ........................................ ..................3 A. Antigen B. Direct vs. Indirect Detection of C. Biotin Signal amplification .

Signal amplification occurs through two mechanisms with these approaches. First, biotinylation (biotin-labeling) typically results in multiple biotin tags per antibody molecule, thus allowing more than one streptavidin molecule to bind to each antibody.

  Amplification