Agar Plate
Found 9 free book(s)Isolation &Characterization of Bacteria
www.gbiosciences.comthe LB agar plate. Gentle press the swab on to the LB agar plate neat one edge. Add 10 l sterile water or PBS to the area where the swab was placed and then streak the plate as described in step 5. 3. Leave the tube at room temperature for 1 minute. 4. Write your group name on the bottom of the agar plate.
Lactobacilli MRS Agar • Lactobacilli MRS Broth
legacy.bd.comthe plate in one direction and then in the reverse direction. 3. Allow the medium to solidify on a flat surface for 5-10 min-utes. 4. Alternatively, plates of Lactobacilli MRS Agar can be used for direct recovery of organisms using the streak inoculation technique. 5. Incubate agar plates at 35°C for 3 days, or at 30°C for 5
SELECTIVE AND DIFFERENTIAL MEDIA
fire.biol.wwu.eduagar plate. Incubate the plate at 37°C for 48 hours. 2. Use a sterile toothpick to scrape tooth enamel. Using the toothpick, make a single short streak on a blood agar plate. Then, using your inoculating loop, streak out the toothpick streaks as illustrated below. Alternatively, you can use
Blood Agar Plates and Hemolysis Protocols
asm.orgAgar 15.0 g Distilled water 1,000 ml Combine the ingredients and adjust the pH to 7.3. Boil to dissolve the agar, and sterilize by autoclaving. Blood Agar To sterile Blood Agar Base which has been melted and cooled to 45 to 50°C, add 5% (vol/vol) sterile defibrinated blood that has been warmed to room temperature.
Streaking Microbial Cultures on Agar Plates
www.umsl.eduAgar streak plates are an essential tool in microbiology. They allow bacteria and fungi to grow ... plate or slant) over the entire surface of a fresh plate. As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Usually by the third
THE IDENTIFICATION OF TWO UNKNOWN BACTERIA
openlab.citytech.cuny.eduagar plate showed that one of the bacteria was a gram-negative rod (Figure 6). This test was deemed useless for identification because all the gram-negative bacteria studied were rods. The unknown gram-positive bacterium was identified as Staphylococcus aureus. This …
BASIC CULTURE TECHNIQUE STREAK PLATE
crcooper01.people.ysu.eduplate. Using the tip of the loop (do not place the loop flat on the agar surface), spread/streak the liquid across the back third of the plate in a smooth back-and-forth motion being sure not to puncture the agar. Also, the back-and-forth strokes should be very close together. Use as much surface area as possible in this portion of the plate.
Luria Broth (LB) and Luria Agar (LA) Media and Their Uses ...
asm.orgStreak Plate for Elucidation of Colony Morphologies Streak a plate of LB agar with a pure or mixed bacterial culture. Incubate inverted plate at organism's ideal temperature for 24 to 48 hours. Observe colony morphology. For more information on colony morphology, see Description of Colonial Morphology of Microorganisms.
Tryptic Soy Agar - BD
legacy.bd.comINSTRUCTIONS FOR USE – PARTIALLY COMPLETED BOTTLED MEDIA BA-256665.02 Rev.: August 2003 BD Tryptic Soy Agar INTENDED USE BD Tryptic Soy Agar, provided in bottles, is a partially completed general purpose medium which, after pouring into Petri dishes or tubes, supports the growth of nonfastidious as well as