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Luria Broth (LB) and Luria Agar (LA) Media and Their Uses ...

Luria Broth (LB) and Luria agar (LA) Media and Their Uses Protocol ||. Created: Monday, 09 October 2006. Author Maria P. MacWilliams Min-Ken Liao Information History LB is a widely used bacterial culture medium today but it has its origins in the field of bacteriophagegenetics. Guiseppi Bertani created the LB. recipe while trying to optimize plaque formation on aShigella indicator strain (Bertani, 1952). According to Bertani, LB has been variously misconstrued to stand for " Luria Broth ", " Luria -Bertani" medium, and "Lennox Broth "; however, the acronym originally stood for "Lysogeny Broth " (Bertani, 2004). The agar form of the medium should be designated LA but it is often referred to as LB. Although originally developed for bacteriophage studies and Shigellagrowth, LB subsequently became the medium of choice for growth of Escherichia coli and other related enteric species.

Streak Plate for Elucidation of Colony Morphologies Streak a plate of LB agar with a pure or mixed bacterial culture. Incubate inverted plate at organism's ideal temperature for 24 to 48 hours. Observe colony morphology. For more information on colony morphology, see Description of Colonial Morphology of Microorganisms.

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