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BASIC CULTURE TECHNIQUE STREAK PLATE

Copyright Chester R. Cooper, Jr. 2022 Microbiology Laboratory (BIOL 3702L) Page 1 of 6 BASIC CULTURE TECHNIQUE : STREAK PLATE Principle and Purpose The isolation of pure cultures of microorganisms is a TECHNIQUE essential to many types of experiments in microbiology as well as in the identification of potential pathogens. One very common way to isolate bacterial and other microbes is by employing the STREAK PLATE TECHNIQUE . In essence, the STREAK PLATE TECHNIQUE is a type of dilution scheme in which a single colony presumably arises from a single cell. In practice, a gradient of cells is established on the surface of an agar PLATE . The result is that confluent growth will occur in one part of the PLATE , but as the as the gradient lessens, single cells will be deposited well separated from other cells.

plate. Using the tip of the loop (do not place the loop flat on the agar surface), spread/streak the liquid across the back third of the plate in a smooth back-and-forth motion being sure not to puncture the agar. Also, the back-and-forth strokes should be very close together. Use as much surface area as possible in this portion of the plate.

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