Example: air traffic controller
Protein Reduction Alkylation Digestion
Found 2 free book(s)Fundamentals of Protein Chemistry - Duke University
genome.duke.eduFor efficient digestion, [protein] of >0.025 μg/mL is required. (Final volume = 4x the volume of urea added)-adjust as necessary. 3. Reduction and alkylation of cysteines: Add 5 μL (or 1/10 volume) of 45 mM DTT. Incubate at 50°C for 15 min. Cool to room temperature. Add 5 μL of 100 mM iodoacetamide.
Protein Reduction, Alkylation, Digestion
proteomicsresource.washington.eduOct 04, 2011 · Protein Reduction, Alkylation, Digestion Last updated 10/4/2011 Page 3 Reduction / Alkylation (DTT, IAA and RapiGest or PPS), Trypsin digestion: Works well for complex mixtures to be analyzed by mass spec. Reagents and Materials (see Table 1) Rapi. Gest SF Powder (Waters Corporation part # 1860018605 Pack of 1 ml Vials,) OR