Transcription of 2.2.25. ABSORPTION SPECTROPHOTOMETRY, …
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2.2.25. ABSORPTION SPECTROPHOTOMETRY, …
EUROPEAN PHARMACOPOEIA ABSORPTION spectrophotometry , ultraviolet and visible IMPURITIES IN GASES. For the analysis of impurities, use a cell transparent to infrared radiation and of suitable optical path length (for example, 1-20 m). Fill the cell as prescribed under Gases. For detection and quantification of the impurities, proceed as prescribed in the monograph. 01/2008:20225. ABSORPTION . spectrophotometry , ultraviolet AND visible . Determination of absorbance. The absorbance (A) of a solution is defined as the logarithm to base 10 of the reciprocal of the transmittance (T) for monochromatic radiation : Figure Typical spectrum of polystyrene used to verify the resolution performance T = I/I0 ;. Verification of the wave-number scale. The wave-number scale may be verified using a polystyrene film, which has I0 = intensity of incident monochromatic radiation ;. transmission minima ( ABSORPTION maxima) at the wave I = intensity of transmitted monochromatic radiation.
2.2.25. Absorption spectrophotometry, ultraviolet and visible EUROPEAN PHARMACOPOEIA 6.0 permitted tolerance is ± 1 nm for the ultraviolet range and ± 3 nm for the visible range.
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