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An Introduction to Fluorescence Spectroscopy

An Introduction to Fluorescence Spectroscopy 2 User Assistance PerkinElmer Ltd Post Office Lane Beaconsfield Buckinghamshire HP9 1QA Printed in the United Kingdom. Notices The information contained in this document is subject to change without notice. PerkinElmer makes no warranty of any kind with regard to the material, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose. PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing, performance or use of this material.

Since the emission of fluorescence always takes place from the lowest vibrational level of the first excited state, the shape of the emission spectrum is always the same, despite changing the wavelength of exciting light. A plot of emission against wavelength for any given excitation wavelength is known as the emission spectrum.

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