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Catalase Test Protocol - American Society for Microbiology

Downloaded from byIP: : Wed, 29 Aug 2018 13:22:48 American Society for Microbiology 2016 1 Catalase Test Protocol | | Created: Thursday, 11 November 2010 Author Karen Reiner Information History In order to survive, organisms must rely on defense mechanisms that allow them to repair or escape the oxidative damage of hydrogen peroxide (H2O2). Some bacteria produce the enzyme Catalase which facilitates cellular detoxification. Catalase neutralizes the bactericidal effects of hydrogen peroxide (13) and its concentration in bacteria has been correlated with pathogenicity (8).

The superoxol catalase test used for the presumptive speciation of certain Neisseria organisms requires a different concentration of H 2O 2. ... was grown on agar containing red blood cells. Carryover of red blood cells into the test may result in a false -positive reaction (5, 7). Using a

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