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CELL CULTURE BASICS - Vanderbilt University

CELL CULTURE BASICS . Cell CULTURE BASICS Handbook B-087243 0110. Contents Introduction .. 1. Purpose of the Handbook .. 1. Introduction to Cell CULTURE .. 2. What is Cell CULTURE ? .. 2. Finite vs Continuous Cell Line .. 2. CULTURE Conditions .. 2. Cryopreservation .. 2. Morphology of cells in CULTURE .. 3. Applications of Cell CULTURE .. 3. Cell CULTURE Laboratory .. 4. Safety .. 4. Biosafety Levels .. 4. SDS .. 5. Safety Equipment .. 5. Personal Protective Equipment (PPE) .. 5. Safe Laboratory Practices .. 5. Cell CULTURE Equipment .. 6. Basic Equipment .. 6. Expanded Equipment .. 6. Additional Supplies .. 6. Cell CULTURE Laboratory .. 7. Aseptic Work Area .. 7. Cell CULTURE Hood .. 7. Cell CULTURE Hood Layout.

Mar 09, 2015 · genetic changes subsequent to the initiation of the parent line. Finite vs Continuous Cell Line Normal cells usually divide only a limited number of times before losing their ability to proliferate, which is a genetically determined event known as senescence; these cell lines are known as finite. However, some cell lines become immortal through ...

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