ch 07 MANUAL PLATELET COUNT - Pearson …

MANUAL PLATELET COUNTP rincipleWhole blood is diluted with a 1% ammonium oxalate solution. The isotonic balance of thediluent is such that all erythrocytes are lysed while the leukocytes, platelets, and reticulocytesremain ,2 The standard dilution for PLATELET counts is 1:100. This dilution is prepared usingthe leukocyte/ PLATELET Unopette The dilution is mixed well and incubated to permit lysisof the erythrocytes. Following the incubation period, the dilution is mounted on ahemacytometer. The cells are allowed to settle and then are counted in a specific area of thehemacytometer chamber under the microscope. The number of platelets is calculated per L (x109/L) of and leukocyte/ PLATELET Unopette reservoirs; each containing mL of the following diluent:Ammonium gSorensen s phosphate buffer gQS with distilled water to 1 Unopette capillary pipets, 20 with cover dish with filter counter6.

MANUAL PLATELET COUNT Principle Whole blood is diluted with a 1% ammonium oxalate solution. The isotonic balance of the diluent is such that all erythrocytes are lysed while the leukocytes, platelets, and reticulocytes

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