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DNA Isolation from Strawberries

The GENETICS Project Department of Genome Sciences University of Washington 1 DNA Isolation from Strawberries Developed by Diane Sweeney Teacher Background This is a simple, effective protocol for spooling DNA. Ripe Strawberries are an excellent source for extracting DNA because they are easy to pulverize and contain enzymes called pectinases and cellulases that help to break down cell walls. And most important, Strawberries have eight copies of each chromosome (they are octoploid), so there is a lot of DNA to isolate.

4. Add 10 ml DNA Extraction Buffer (soapy salty water) and squish for a few more minutes. Try not to make a lot of soap bubbles. 5. Filter through a moistened paper towel set in a funnel, and collect the liquid in a clear tube. Do not squeeze the paper towel. Collect about 3 ml liquid. 6.

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