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Part# 9PIM180 Revised 4/18

Certificate of AnalysisQuality Control AssaysPromega Corporation2800 Woods Hollow RoadMadison, WI 53711-5399 USAT elephone608-274-4330 Toll USE LIMITATIONS, WARRANTY, DISCLAIMERP romega manufactures products for a number ofintended uses. Please refer to the product label for theintended use statements for specific products contain chemicals which may beharmful if misused. Due care should be exercised withall Promega products to prevent direct human Promega product is shipped with documentationstating specifications and other technical products are warranted to meet or exceed thestated specifications. Promega's sole obligation and thecustomer's sole remedy is limited to replacement ofproducts free of charge in the event products fail toperform as warranted.

Tris-HCl (pH 7.8), 100mM MgCl 2, 100mM DTT and 10mM ATP. The performance of this buffer depends on the integrity of the ATP. Store the buffer in small aliquots at –20°C to minimize degradation of the ATP and DTT. Note: The DTT in the Ligase 10X Buffer may precipitate upon freezing. If this occurs, vortex the buffer until the precipitate

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Transcription of Part# 9PIM180 Revised 4/18

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Temperature Dependence of pH for Commonly Used Buffers.

www.promega.com

TECHNICAL REFERENCE PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD MADISON, WI 53711-5399 USA TELEPHONE 608-274-4330 www.promega.com ©2010 ALL RIGHTS RESERVED PART #GE647 Temperature Dependence of pH for Commonly Used Buffers

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pGEM -T and pGEM -T Easy Vector Systems - Promega

www.promega.com

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Certificate of Analysis GoTaq Part# 9PIM712

www.promega.com

Certificate of Analysis GoTaq® Green Master Mix Cat.# Size M7122 100 reactions M7123 1,000 reactions Cat.# M7122 and M7123 include GoTaq® Green Master Mix, 2X, and Nuclease-Free Water. Description: GoTaq® Green Master Mix(a) is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal …

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AMP-Glo Assay Technical Manual TM384 - Promega

www.promega.com

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NanoLuc®: A Smaller, Brighter, and More ... - Promega

www.promega.com

No post-translational modifications detected in mammalian cells No disulfide bonds ... Experimental details: transient transfection of HEK293 cells with NF-kB inducible constructs. rhTNFa treatment for 5 hours. 0.001 0.01 0.1 1 10 100 10 4 10 5 10 6 10 7 10 8 10 9 10 10 FlucP NlucP Fluc NLuc

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CellTiter-Glo Luminescent Cell Viability Assay Technical ...

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washing, removal of medium or multiple pipetting steps are not required. ... Each vial of substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000 assays at 25µl/assay in 384-well plates (10,000 to 40,000 total assays). Includes:

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Dual-Luciferase Reporter Assay System - Promega

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Dual reporters are commonly used to improve experimental accuracy. The term “dual reporter” refers to the simultaneous expression and measurement of two individual reporter enzymes within a single system. Typically, the

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pGL4 Luciferase Reporter Vectors - Promega

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The Pico DNA Quantitation Using PicoGreen

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Final DNA Concentration in PicoGreen Assay 1000 0 1000 1000 ng/mL 500 500 1000 500 ng/mL 100 900 1000 100 ng/mL 10 990 1000 10 ng/mL 1 999 1000 1ng/mL 0 1000 1000 blank Table 1. Protocol for preparing standard curve. 3.2.3 After incubation calibrate the Picofluor Fluorometer. Press “STD VAL” and enter “999” for the highest standard ...

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webhome.auburn.edu

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www.chem.konan-u.ac.jp

4.2 Tris 生理学的緩衝液で最も使われてきたのはTrisH2NC(CH2OH)3 (tris(hydroxymethyl)aminomethane) である。緩衝作用に関連した反応は [H3NC(CH2OH)3] + H+ +H 2NC(CH2OH)3 (27) で、解離のpKa は8.08である。pKa より酸性側で使用するため通常は塩酸と組み合わせる。以

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www.gs.washington.edu

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RT-PCR: Two-Step Protocol - MIT OpenCourseWare

ocw.mit.edu

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