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Gram Stain Protocols

Downloaded from byIP: : Mon, 12 Aug 2019 17:45:19 American Society for Microbiology 2016 1 gram Stain Protocols | | Created: Friday, 30 September 2005 Author Ann C. Smith Marise A. Hussey Information History The gram Stain was first used in 1884 by Hans Christian gram ( gram ,1884). gram was searching for a method that would allow visualization of cocci in tissue sections of lungs of those who had died of pneumonia. Already available was a staining method designed by Robert Koch for visualizing turbercle bacilli. gram devised his method that used Crystal Violet (Gentian Violet) as the primary Stain , an iodine solution as a mordant followed by treatment with ethanol as a decolorizer.

the solution as iodine oxidizes (McClelland, 2001) and uses an aqueous solution of Safranin for the counterstain (Gephardt et al., 1981). The reagents listed below can be made or purchased commercially from biological supply houses 1. Primary Stain: Crystal Violet Staining Reagen t. Solution A for crystal violet staining reagent

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